![Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NB100-1406-img0004.jpg "Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence SRCGELLPLIDPC corresponding to C-Terminus according to NP_036372.1. |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SIRT4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 37 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa band according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human pancreas shows staining of the islets of langerhans. IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SIRT4 |
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![Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NB100-1406-img0001.jpg "Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NB100-1406-img0003.jpg "Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
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