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Shugoshin Antibody (3C11)

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Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: ...read more
Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 transfected lysate using anti-SGOL1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGOL1 MaxPab rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, IP
Clone
3C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Shugoshin Antibody (3C11) Summary

Immunogen
SGOL1 (AAH17867, 1 a.a. ~ 292 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALENEKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQIPLEETELPGQGESFQIEATPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKRALEVSPAKEAIFILYYVREFVSRFPDCRKCKLETHICLR
Localization
Nuclear. Localizes to the centromere. BUB1 is required for centromeric localization.
Specificity
SGOL1 - shugoshin-like 1 (S. pombe)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SGO1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26670547).
Publications
Read Publications using H00151648-M01.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Shugoshin Antibody (3C11)

  • FLJ14230
  • hSgo1
  • NY-BR-85
  • Serologically defined breast cancer antigen NY-BR-85
  • SGO
  • Sgo1
  • shugoshin 1AB protein
  • shugoshin 1CD protein
  • shugoshin 1EF protein
  • shugoshin 1GH protein
  • shugoshin 1KL protein
  • shugoshin-like 1 (S. pombe)
  • shugoshin-like 1

Background

Shugoshin (Sgo) proteins constitute a conserved protein family defined as centromeric protectors of Rec8-containing cohesin complexes in meiosis. It has been demonstrated that vertebrate shugoshin is required for preserving centromeric cohesion during mitosis. The presence of human Scc1 at centromeres in mitosis is dependent on human Sgo1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Shugoshin Antibody
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H00151648-M01
Shugoshin Antibody (3C11)
Species: Hu, Mu, Rt
Applications: WB, ELISA, Func, ICC/IF, IHC, IP

Publications for Shugoshin Antibody (H00151648-M01)(18)

We have publications tested in 2 applications: ICC/IF, WB.


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(1)
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(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using H00151648-M01 Applications Species
Qinfu C, Miao Z, Xuan P et al. Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation. Cell Rep. 2021-09-21 [PMID: 34551298]
Zhang M, Liang C, Chen Q et al. Histone H2A phosphorylation recruits topoisomerase II? to centromeres to safeguard genomic stability. EMBO J. 2019-11-26 [PMID: 31769059]
Vallardi G, Allan LA, Crozier L, Saurin AT et al. Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. Elife. 2019-03-04 [PMID: 30829571]
Liang C, Zhang Z, Chen Q et al. A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells. J Biol Chem. 2018-11-29 [PMID: 30498087]
Song AT, Galli A, Leclerc S et al. Dataset of Sgo1 expression in cardiac, gastrointestinal, hepatic and neuronal tissue in mouse. Data Brief 2017-07-01 [PMID: 28748208]
Zielinska AP, Holubcova Z, Blayney M et al. Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect. Elife 2015-12-15 [PMID: 26670547] (ICC/IF) ICC/IF
Asghar A, Lajeunesse A, Dulla K et al. Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation. Nat Commun 2015-09-24 [PMID: 26399325] (WB) WB
C. van de Werken1, M. Avo Santos, J.S.E. Laven et al. Chromosome segregation regulation in human zygotes: altered mitotic histone phosphorylation dynamics underlying centromeric targeting of the chromosomal passenger complex. Hum Reprod. 2015-07-29 [PMID: 26223676]
Nijenhuis W, Vallardi G, Teixeira A et al. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014-11-17 [PMID: 25402682]
Garcia-Cruz R, Brieno MA, Roig I et al. Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes. Hum Reprod. 2010-07-15 [PMID: 20634189]
Show All 18 Publications.

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Bioinformatics

Gene Symbol SGO1
Entrez
OMIM
Uniprot