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Aurora B Antibody - BSA Free

Images

 
Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 ...read more
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, MA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
12.5 mg/ml

Order Details

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Aurora B Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4
Marker
Mitosis Marker
Specificity
Aurora B
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 50-60 ug
  • Microarray
  • Western Blot 1:1000-1:2000
Application Notes
By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Aurora B Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB100-294 in the following applications:

Publications
Read Publications using
NB100-294 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
12.5 mg/ml
Purity
IgG purified

Alternate Names for Aurora B Antibody - BSA Free

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora B Antibody (NB100-294)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.


Filter By Application
ICC/IF
(6)
IF/IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-294 Applications Species
Chang X, Zhang T, Wang Q et al. HI-511 overcomes melanoma drug resistance via targeting AURKB and BRAF V600E Theranostics 2020-08-01 [PMID: 32863956] (Human) Human
Carlotta Penzo, Laura Arnoldo, Silvia Pegoraro, Sara Petrosino, Gloria Ros, Rossella Zanin, Jacek R. Wiśniewski, Guidalberto Manfioletti, Riccardo Sgarra HMGA1 Modulates Gene Transcription Sustaining a Tumor Signalling Pathway Acting on the Epigenetic Status of Triple-Negative Breast Cancer Cells Cancers 2019-08-02 [PMID: 31382504]
Fang H, Niu K, Mo D et al. RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity. Oncogenesis 2018-09-12 [PMID: 30206236] (ICC/IF, Human) ICC/IF Human
Dumitru AMG, Rusin SF, Clark AEM et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability Elife. 2017-11-20 [PMID: 29154753] (ICC/IF, WB, Human)

Details:
HEC1 IF antibody used at a dilution of 1:500
ICC/IF, WB Human
Sijare F, Geibler AL, Fichter CD et al. Aurora B expression and histone variant H1.4S27 phosphorylation are no longer coordinated during metaphase in aneuploid colorectal carcinomas Virchows Arch. 2015-02-14 [PMID: 25680570] (ICC/IF) ICC/IF
Kleyman M, Kabeche L, Compton DA. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J. Cell. Sci. 2014-07-29 [PMID: 25074805] (ICC/IF, Human)

Details:
Outer kinetochore component Hec1/NDC80 antibody used in ICC-IF on HCT116 and RPE1 cells for measuring centromere stretch (Fig. 1D, supplementary material Fig. S2A)
ICC/IF Human
Kabeche L, Compton DA. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013-10-03 [PMID: 24013174] (ICC/IF, Human) ICC/IF Human
Kabeche L, Compton DA. Checkpoint-independent stabilization of kinetochore-microtubule attachments by Mad2 in human cells. Curr Biol 2012-04-01 [PMID: 22405866] (ICC/IF, Human) ICC/IF Human
Beussel S, Hasenburg A, Bogatyreva L, Hauschke D, Werner M, Lassmann S. Aurora-B protein expression is linked to initial response to taxane-based first-line chemotherapy in stage III ovarian carcinoma. J Clin Pathol;65(1):29-35. 2012-01-01 [PMID: 22011448] (IF/IHC, Human) IF/IHC Human
Bufo P, Sanguedolce F, Tortorella S et al. Expression of mitotic kinases phospho-aurora A and aurora B correlates with clinical and pathological parameters in bladder neoplasms. Histol Histopathol;25(11):1371-7. 2010-11-01 [PMID: 20865660]
Show All 12 Publications.

Reviews for Aurora B Antibody (NB100-294) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using NB100-294:
Filter by Applications
IF
(3)
IHC-P
(1)
ICC
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Aurora B NB100-294
Enlarge
5
reviewed by:
Verified Customer
IF Human 04/21/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE1 cells
SpeciesHuman
Immunohistochemistry-Paraffin Aurora B NB100-294
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 10/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested293T cell line
SpeciesHuman
Immunocytochemistry Aurora B NB100-294
Enlarge
4
reviewed by:
Andrew Cosgrove
ICC Human 11/06/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedRPE-1 cells
SpeciesHuman
  5
reviewed by:
Verified Customer
IF Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
  5
reviewed by:
Duane Compton
IF Human 05/02/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE-1
SpeciesHuman
Lot043K4827

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora B Antibody (NB100-294) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Aurora B Antibody (NB100-294)

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Blogs on Aurora B

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Calculators

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Verified Customer
04/21/2016
Application: IF
Species: Human

Verified Customer
10/02/2015
Application: IHC-P
Species: Human

Andrew Cosgrove
11/06/2013
Application: ICC
Species: Human

Bioinformatics

Gene Symbol AURKB
Entrez