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SHP/NR0B2/Nuclear Receptor SHP Antibody

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Western Blot: SHP/NR0B2/Nuclear Receptor SHP Antibody [NBP1-33597] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: SHP/NR0B2/Nuclear Receptor SHP Antibody [NBP1-33597] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NBP1-33597] - SW480 Xenograft, using NR0B2(GTX113874) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SHP/NR0B2/Nuclear Receptor SHP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SHP/NR0B2/Nuclear Receptor SHP. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR0B2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SHP/NR0B2/Nuclear Receptor SHP Antibody

  • FLJ17090
  • NR0B2
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • Orphan nuclear receptor SHP
  • SHP
  • SHPSHP1
  • Small heterodimer partner

Background

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SHP/NR0B2/Nuclear Receptor SHP Antibody (NBP1-33597) (0)

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NR0B2