![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody-[Biotin]-Immunocytochemistry-Immunofluorescence-NB300-229B-img0002.jpg "Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.")
| Reactivity | Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)Species Glossary |
| Applications | WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Immunogen | A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209] |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SYCP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
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| Readout System | ||
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| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
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![SCP1 Antibody [Biotin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "SCP1 Antibody [Biotin]")
| Publication using NB300-229B | Applications | Species |
|---|---|---|
| Lam KG, Brick K, Cheng G et al Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis. Nat Commun. 2019-08-23 [PMID: 31444359] (ICC/IF, Mouse) Details: Citation using the Biotin version of this antibody. |
ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for SCP1 Antibody (NB300-229B)Find related products by research area.
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Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati... Read full blog post. |
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SCP1 a Potential Cancer Target for Immunotherapy Synaptonemal Complex Protein 1 (SCP1) is a novel tumor antigen that belongs to the growing family of cancer/testis antigens (CTA). SCP-1 is known to be selectively expressed during the meiotic prophase of spermatocytes and is involved in the pairing o... Read full blog post. |
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| Gene Symbol | SYCP1 |
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody-[Biotin]-Immunocytochemistry-Immunofluorescence-NB300-229B-img0001.jpg "Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images & signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t & SCP1, another one using SCP3 & STRA8. *Early & late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, & are therefore merged for counting & signal quantification. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31444359), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-229b_rabbit-polyclonal-scp1-antibody-biotin-31020241534383.jpg "Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images & signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t & SCP1, another one using SCP3 & STRA8. *Early & late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, & are therefore merged for counting & signal quantification. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31444359), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")