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SATB1 Antibody (OTI13D6) - Azide and BSA Free

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Western Blot: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1 mouse monoclonal antibody.
Western Blot: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI13D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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SATB1 Antibody (OTI13D6) - Azide and BSA Free Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SATB1 expression vector
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SATB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SATB1 Antibody (OTI13D6) - Azide and BSA Free

  • DNA-binding protein SATB1
  • SATB homeobox 1
  • SATB1
  • special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
  • Special AT-rich sequence-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SATB1