![Western Blot: SATB1 Antibody (13D6) [NBP1-47950] Analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Western-Blot-NBP1-47950-img0011.jpg "Western Blot: SATB1 Antibody (13D6) [NBP1-47950] Analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI13D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length protein expressed in 293T cell transfected with human SATB1 expression vector |
| Specificity | This antibody is specific for Homo sapiens SATB homeobox 1 (SATB1), transcript variant 1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SATB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 86 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||
|---|---|---|---|---|---|---|---|
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reviewed by:
Ranveer Singh |
WB | 05/22/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: SATB1 Antibody (13D6) [NBP1-47950] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunocytochemistry-Immunofluorescence-NBP1-47950-img0010.jpg "Immunocytochemistry/Immunofluorescence: SATB1 Antibody (13D6) [NBP1-47950] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0009.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1 mouse monoclonal antibody.")
![Western Blot: SATB1 Antibody (13D6) [NBP1-47950] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Western-Blot-NBP1-47950-img0001.jpg "Western Blot: SATB1 Antibody (13D6) [NBP1-47950] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0002.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0003.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0004.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0005.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0006.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0007.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SATB1-Antibody-13D6-Immunohistochemistry-Paraffin-NBP1-47950-img0008.jpg "Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1 mouse monoclonal antibody.")
![Western Blot IRF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/IRF1_AF4715_Western_Blot_5529.jpg)
![Western Blot IRF1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af4715_mouse-irf1-affinity-purified-polyclonal-ab-western-blot-16122024101037..png)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Western Blot GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Western_Blot_5199.jpg)
![Immunocytochemistry GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Immunocytochemistry_6486.jpg)
![Simple Western GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA3_AF2605_Simple_Western_16988.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")