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S5a/Angiocidin Antibody

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Western Blot: Proteasome 19S S5A Antibody [NBP2-19951] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: Hep G2 D: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: Proteasome 19S S5A Antibody [NBP2-19951] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: Hep G2 D: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19951] - SK-N-SH cells. Green: PSMD4 protein stained by PSMD4. Blue: Hoechst 33342 staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb, RM, Sh, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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S5a/Angiocidin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human S5a/Angiocidin. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Porcine (100%), Bovine (99%), Rabbit (98%), Rhesus Macaque (100%), Chicken (97%), Canine (99%), Sheep (100%), Primate (100%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD4
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%), Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for S5a/Angiocidin Antibody

  • 26S proteasome non-ATPase regulatory subunit 4;AF;AF-1;ASF;MCB1;PSMD4;pUB-R5;Rpn10;S5A
  • Angiocidin
  • ASF
  • Macropain
  • Mcb1
  • PSMD4
  • pUB-R5
  • Rpn10
  • S5a

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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S5a/Angiocidin
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NBP2-19951
S5a/Angiocidin Antibody
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb, RM, Sh, Xp
Applications: WB, ICC/IF
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMD4
Uniprot