![Western Blot: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1481).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Western-Blot-NBP2-54580-img0013.jpg "Western Blot: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1481).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, MA, CyTOF-ready |
| Clone | S100A4/1481 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53178). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary) (Uniprot: P26447) |
| Localization | Nucleus, Cytoplasm |
| Marker | Marker of Tumor Metastasis |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S100A4 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
| Publication using NBP2-54580 | Applications | Species |
|---|---|---|
| Hellinger JW, HUchel S, Goetz L et al. Inhibition of CYR61-S100A4 Axis Limits Breast Cancer Invasion Front Oncol. 2019-10-23 [PMID: 31709177] (Human) Details: Citation used the Alexa Fluor 488 format of this antibody. |
Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for S100A4 Antibody (NBP2-54580)Find related products by research area.
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | S100A4 |
![Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Immunofluorescence staining of HeLa cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54580-img0012.jpg "Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Immunofluorescence staining of HeLa cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Formalin-paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1481).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54580-img0001.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Formalin-paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1481).")
![Flow (Intracellular): S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1481) NBP2-54580PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Flow-Intracellular-NBP2-54580-img0011.jpg "Flow (Intracellular): S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1481) NBP2-54580PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.")
![Western Blot: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1481).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Western-Blot-NBP2-54580-img0002.jpg "Western Blot: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1481).")
![Flow Cytometry: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Flow Cytometric Analysis of A549 cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54580-img0009.jpg "Flow Cytometry: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Flow Cytometric Analysis of A549 cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Protein Array: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Antibody (S100A4/1481).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1481-Azide-and-BSA-Free-Protein-Array-NBP2-54580-img0016.jpg "Protein Array: S100A4 Antibody (S100A4/1481) - Azide and BSA Free [NBP2-54580] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Antibody (S100A4/1481).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")