Home » RTN4IP1 » RTN4IP1 Lysates » RTN4IP1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

RTN4IP1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: RTN4IP1 Overexpression Lysate (Adult Normal) [NBL1-15613] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RTN4IP1.
Western Blot: RTN4IP1 Overexpression Lysate (Adult Normal) [NBL1-15613] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RTN4IP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

RTN4IP1 Overexpression Lysate Summary

Description

RTN4IP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202957

Accession#: NM_032730

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
RTN4IP1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for RTN4IP1 Overexpression Lysate

  • MGC12934
  • NIMPNOGO-interacting mitochondrial protein
  • reticulon 4 interacting protein 1
  • reticulon-4-interacting protein 1, mitochondrial

Background

This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

UQCRC2 Antibody
NBP1-80861
Immunocytochemistry/ Immunofluorescence UQCRC2 Antibody
Immunohistochemistry-Paraffin UQCRC2 Antibody
Immunohistochemistry-Paraffin UQCRC2 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
UQCRC1 Antibody
(OTI1G6)
NBP2-03825
Flow Cytometry UQCRC1 Antibody (OTI1G6)
Immunohistochemistry-Paraffin UQCRC1 Antibody (OTI1G6)
Immunohistochemistry-Paraffin UQCRC1 Antibody (OTI1G6)
(3 Publications)
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
Nogo Antibody
NB100-56681
Immunocytochemistry/ Immunofluorescence Nogo Antibody
Simple Western Nogo Antibody
Immunohistochemistry-Paraffin Nogo Antibody
(23 Publications)
Species: Hu, Mu, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
Angiopoietin-2
[HRP]
DANG20
N/A Angiopoietin-2 [HRP]
N/A Angiopoietin-2 [HRP]
(103 Publications)
Species: Hu
Applications: ELISA

Acetylcholinesterase/A
NB100-1519
Flow Cytometry Acetylcholinesterase/ACHE Antibody
Western Blot Acetylcholinesterase/ACHE Antibody
Immunocytochemistry/ Immunofluorescence Acetylcholinesterase/ACHE Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB

Butyrylcholinesterase/ ...
AF9024
Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]
Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]
(2 Publications)
Species: Mu, Rt
Applications: IHC, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(453 Publications)
Species: Hu
Applications: BA
Prolactin Antibody
[Unconjugated]
AF1445
Neutralization Prolactin Antibody [Unconjugated]
Western Blot Prolactin Antibody [Unconjugated]
Immunohistochemistry Prolactin Antibody [Unconjugated]
(7 Publications)
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
Cytokeratin 17
Antibody
NBP2-38536
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Coagulation Factor
XIV/Protein C Antibod ...
AF4885
Coagulation Factor XIV/Protein C Antibody
(2 Publications)
Species: Mu
Applications: IP, WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
Bioactivity TGF-beta 1 [Unconjugated]
(53 Publications)
Species: Hu
Applications: BA
Angiopoietin-1
[Unconjugated]
923-AN
Angiopoietin-1 [Unconjugated]
(71 Publications)
Species: Hu
Applications: BA
Integrin beta
2/CD18 Antibody [Unco ...
AF1730
Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]
Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
Podocalyxin Like
Antibody [Unconjugate ...
AF1556
Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]
Western Blot Podocalyxin Like Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]
(3 Reviews)
(100 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
SLC25A1 Antibody
NBP1-31851
Western Blot SLC25A1 Antibody
Western Blot SLC25A1 Antibody
Western Blot SLC25A1 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Lactoperoxidase
Antibody - BSA Free
NB110-61574
Western Blot Lactoperoxidase Antibody - BSA Free
Species: Bv, Gt, Hu, Mu, Sh
Applications: WB

IL-1ra/IL-1F3/IL1RN [H
DRA00B
N/A IL-1ra/IL-1F3/IL1RN [HRP]
N/A IL-1ra/IL-1F3/IL1RN [HRP]
N/A IL-1ra/IL-1F3/IL1RN [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
P-Selectin/CD62P
137-PS
P-Selectin/CD62P
(46 Publications)
Species: Hu
Applications: BA
RTN4IP1
Overexpression Lysate
NBL1-15613
RTN4IP1 Overexpression Lysate
Species: Hu
Applications: WB

Publications for RTN4IP1 Lysate (NBL1-15613) (0)

There are no publications for RTN4IP1 Lysate (NBL1-15613).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RTN4IP1 Lysate (NBL1-15613) (0)

There are no reviews for RTN4IP1 Lysate (NBL1-15613). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RTN4IP1 Lysate (NBL1-15613) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RTN4IP1 Products

Blogs on RTN4IP1

There are no specific blogs for RTN4IP1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RTN4IP1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol RTN4IP1