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Lactoperoxidase Antibody - BSA Free

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Western Blot: Lactoperoxidase Antibody [NB110-61574] - Detection of LPO in MDA-MB231 cell lysate

Product Details

Summary
Reactivity Hu, Mu, Bv, Gt, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.5 mg/ml

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Lactoperoxidase Antibody - BSA Free Summary

Background
Lactoperoxidase is a member of the heme peroxidase family and is secreted from mammary, salivary, and other mucosal glands. Lactoperoxidase is involved in aiding the process to catalyze the oxidation of several organic and inorganic substrates by H2O2. These oxidized products produced through the action of Lactoperoxidase have potent bactericidal activities. A natural antibacterial produce...r, Lactoperoxidase has potential to be used in controlling bacteria in food and other health care products
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Immunogen
Synthetic peptide made to a C-terminal portion of the human Lactoperoxidase protein (within residues 650-712). [Swiss-Prot# P22079]
Localization
Secreted, extracellular space.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Application Notes
This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactoperoxidase Antibody - BSA Free

  • EC 1.11.1
  • lactoperoxidase
  • MGC129990
  • MGC129991
  • Salivary peroxidase
  • SAPX
  • SPOEC 1.11.1.7

Background

Lactoperoxidase is an antibacterial agent in cow milk. The heme protein lactoperoxidase (LPO), also referred to as salivary peroxidase (SPO), is an oxidoreductase secreted into milk. LPO belongs to the XPO subfamily of the peroxidase family. It is expressed in mammary and salivary glands, and in the presence of H2O2, LPO acts as a catalyst for the oxidation of many phenols and aromatic amines. It is crucial for protecting the lactating mammary gland and intestinal tract of newborn infants against microorganisms. LPO binds one calcium ion per heterodimer and one heme B (iron-protoporphyrin IX) group covalently per heterodimer. The LPO gene, which spans 28 kb, is similar in gene organization and sequence to the peroxidase genes MPO and EPX, suggesting the possibility that these genes evolved from a common ancestral gene. The LPO and MPO genes are arranged in a tail-to-tail manner on chromosome 17q23.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LPO