Independent Antibodies: Western Blot: RTCD1 Antibody [NBP1-87113] - Analysis using Anti-RTCA antibody NBP1-87113 (A) shows similar pattern to independent antibody NBP2-58714 (B).
Immunocytochemistry/ Immunofluorescence: RTCD1 Antibody [NBP1-87113] - Staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: RTCD1 Antibody [NBP1-87113] - Staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.
Western Blot: RTCD1 Antibody [NBP1-87113] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
This antibody was developed against Recombinant Protein corresponding to amino acids: LFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTCA
Purity
Immunogen affinity purified
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IHC, WB reported in scientific literature (PMID: 25961792). For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
Drosophila reactivity reported in scientific literature (PMID: 25961792).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for RTCD1 Antibody
phosphate cyclase
RNA 3'-terminal phosphate cyclase
RNA cyclase
RNA terminal phosphate cyclase domain 1
RNA terminal phosphate cyclase domain-containing protein 1
RNA-3'-phosphate cyclase
RPC1
RPCEC 6.5.1.4
RTC domain containing 1
RTC domain-containing protein 1
RTC1
Background
RNA 3-prime-terminal phosphate cyclase (RPC; EC 6.5.1.4) catalyzes the ATP-dependent conversion of a 3-prime phosphate to a 2-prime,3-prime-cyclic phosphodiester at the end of RNA (Genschik et al., 1997 [PubMed 9184239]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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