![Western Blot: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug) Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Azide-and-BSA-Free-Western-Blot-NBP2-80929-img0001.jpg "Western Blot: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug) Image from the standard format of this antibody.")
| Reactivity | Hu, Ha, Pm, Mu(-), Rt(-)Species Glossary |
| Applications | WB, ELISA, IHC, KO |
| Clone | 2E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823] |
| Localization | Cytoplasmic and Mitochondrial. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNASEL |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 83 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for RNase L Antibody (NBP2-80929)Find related products by research area.
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| Gene Symbol | RNASEL |
![Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80929-img0002.jpg "Immunohistochemistry: RNase L Antibody (2E9) - Azide and BSA Free [NBP2-80929] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
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