![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
| Reactivity | Hu, Ce, ZeSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, ChIP, KD |
| Clone | C.15200005 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | The exact immunogen is propietary information. |
| Modification | p Ser2 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLR2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005)")
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reviewed by:
Verified Customer |
WB | Human | 05/01/2024 |
Summary
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reviewed by:
Wendy Lyu |
ChIP | Human | 09/11/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for RNA Polymerase II/POLR2A Antibody (NBP2-59215)Find related products by research area.
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| Verified Customer 05/01/2024 |
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| Application: | WB | |
| Species: | Human |
| Wendy Lyu 09/11/2020 |
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| Application: | ChIP | |
| Species: | Human |
| Gene Symbol | POLR2A |
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Chromatin-Immunoprecipitation-NBP2-59215-img0004.jpg "Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-ELISA-NBP2-59215-img0003.jpg "ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.")
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Immunofluorescence-NBP2-59215-img0005.jpg "Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
