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RhoGAP Antibody

Images

 
Western Blot: RhoGAP Antibody [NBP2-20158] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: RhoGAP Antibody [NBP2-20158] - Immunohistochemical analysis of paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RhoGAP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RhoGAP. The exact sequence is proprietary.
Localization
Cytoplasm; Membrane; Peripheral membrane protein
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGAP5
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for RhoGAP Antibody

  • growth factor independent 2
  • p100 RasGAP-associated p105 protein
  • p105 RhoGAP
  • p190-BGFI2
  • p190BRhoGAP
  • Rho GTPase activating protein 5
  • rho GTPase-activating protein 5
  • RHOGAP5
  • Rho-type GTPase-activating protein 5

Background

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGAP5
Uniprot