Canine Renin ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates and other biological fluids
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
5.9 pg/mL

Order Details

Canine Renin ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
5.9 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
78 - 107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
REN, Ren1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Canine Renin ELISA Kit (Colorimetric)

  • angiotensin-forming enzyme
  • Angiotensinogenase
  • EC 3.4.23
  • EC 3.4.23.15
  • FLJ10761
  • HNFJ2
  • Prorenin
  • REN
  • renin precursor, renal
  • Renin

Background

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol REN, Ren1