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Rat Renin ELISA Kit (Colorimetric)

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ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Samples were spiked with high concentrations of Rat Renin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.625-1000 pg/mL
Sensitivity
9.375 pg/mL

Order Details

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Rat Renin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
Standard Curve Range
15.625-1000 pg/mL
Sensitivity
9.375 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.04%
Intra-Assay
CV% < 5.13%
Spike Recovery
88-110%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
REN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Renin ELISA Kit (Colorimetric)

  • angiotensin-forming enzyme
  • Angiotensinogenase
  • EC 3.4.23
  • EC 3.4.23.15
  • FLJ10761
  • HNFJ2
  • Prorenin
  • REN
  • renin precursor, renal
  • Renin

Background

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol REN
Uniprot