Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Rat TNF RI is a 461 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa extracellular domain (ECD) with a PLAD (pre‑ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 227 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of rat TNF RI shares 69%, 88%, 66%, 68% and 75% aa sequence identity with human, mouse, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF‑ alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF‑ beta /TNFSF1B (lymphotoxin‑ alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF‑ alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8  9, 16). Release of the 28‑34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (13). Release helps to resolve inflammatory reactions, since it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR‑associated periodic syndrome) (7).

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioassay.

Showing Publication 1 - 1 of 1.

Publication using 7990-TR	Applications	Species
Clark A, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold K, Malcangio M, Sandkuhler J Selective activation of microglia facilitates synaptic strength. J Neurosci, 2015-03-18;35(11):4552-70. 2015-03-18 [PMID: 25788673] (Bioassay, Rat)	Bioassay	Rat

Array 7990-TR

• TNF RI/TNFRSF1A Antibodies
• TNF RI/TNFRSF1A Antibody Pairs
• TNF RI/TNFRSF1A ELISA Kits
• TNF RI/TNFRSF1A Lysate
• TNF RI/TNFRSF1A Proteins
• TNF RI/TNFRSF1A ELISAs
• TNF RI/TNFRSF1A Proteins and Enzymes