Recombinant Rat CNTF Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems’ cytokine catalog.

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian, Chicken, Zebrafish.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

Showing Publications 1 - 10 of 36. Show All 36 Publications. Collapse Publications.

Publications using 557-NT	Applications	Species
EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798415] (Bioassay, Mouse)	Bioassay	Mouse
Y Tang, DJ Kwiatkowsk, EP Henske Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment Nature Communications, 2022-08-26;13(1):5018. 2022-08-26 [PMID: 36028490] (Bioassay, Mouse)	Bioassay	Mouse
S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 2022-08-25;13(1):5003. 2022-08-25 [PMID: 36008413] (Bioassay, Mouse)	Bioassay	Mouse
G D'Este, M Stazi, S Negro, A Megighian, F Lista, O Rossetto, C Montecucco, M Rigoni, M Pirazzini Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration International Journal of Molecular Sciences, 2022-01-21;23(3):. 2022-01-21 [PMID: 35163106] (Bioassay, Rat)	Bioassay	Rat
KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020-10-21;0(0):. 2020-10-21 [PMID: 33091368] (Bioassay, Mouse)	Bioassay	Mouse
KM Collura, J Niu, SS Sanders, A Montersino, SM Holland, GM Thomas The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem., 2020-09-21;0(0):. 2020-09-21 [PMID: 32958558] (Bioassay, Rat)	Bioassay	Rat
F Bucher, E Aguilar, KV Marra, J Rapp, J Arnold, S Diaz-Aguil, C Lange, H Agostini, G Schlunck, A Stahl, M Friedlande CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10 Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):20. 2020-08-03 [PMID: 32780864] (In Vivo, Mouse)	In Vivo	Mouse
C Soulard, C Salsac, K Mouzat, C Hilaire, J Roussel, A Mezghrani, S Lumbroso, C Raoul, F Scamps Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis Cell Rep, 2020-02-25;30(8):2581-2593.e7. 2020-02-25 [PMID: 32101737] (Bioassay, Mouse)	Bioassay	Mouse
H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder Cell Rep, 2019-12-24;29(13):4362-4376.e6. 2019-12-24 [PMID: 31875546] (Cell Culture, Mouse)	Cell Culture	Mouse
H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019-07-10;0(0):. 2019-07-10 [PMID: 31292217] (Cell Culture, Rat)	Cell Culture	Rat
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019-03-14;9(1):4515. 2019-03-14 [PMID: 30872638] (Bioassay, Mouse)	Bioassay	Mouse
C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019-01-29;26(5):1143-1156.e5. 2019-01-29 [PMID: 30699345] (Bioassay, Mouse)	Bioassay	Mouse
J Di Pierdom, R Scholz, FJ Valiente-S, MC Sánchez-Mi, M Vidal-Sanz, T Langmann, M Agudo-Barr, D García-Ayu, MP Villegas-P Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration Invest. Ophthalmol. Vis. Sci., 2018-09-04;59(11):4392-4403. 2018-09-04 [PMID: 30193320] (In Vivo, Rat)	In Vivo	Rat
KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018-05-22;9(6):596. 2018-05-22 [PMID: 29789529] (Bioassay, Mouse)	Bioassay	Mouse
R Rajagopal, S Zhang, X Wei, T Doggett, S Adak, J Enright, V Shah, G Ling, S Chen, J Yoshino, FF Hsu, CF Semenkovic Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision JCI Insight, 2018-01-11;3(1):. 2018-01-11 [PMID: 29321376] (Bioassay, Rat)	Bioassay	Rat
SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins Front Pharmacol, 2017-02-23;8(0):73. 2017-02-23 [PMID: 28280466] (Bioassay, Mouse)	Bioassay	Mouse
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493. 2017-03-07 [PMID: 28270618] (Bioassay, Mouse)	Bioassay	Mouse
CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 2016-08-01;57(10):4017-26. 2016-08-01 [PMID: 27494343] (Bioassay, Human)	Bioassay	Human
A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016-05-25;0(0):. 2016-05-25 [PMID: 27226316] (Bioassay, Rat)	Bioassay	Rat
Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 2015-06-17;10(6):e0129944. 2015-06-17 [PMID: 26083449] (Bioassay, Mouse)	Bioassay	Mouse
Hong S, Song M Signal transducer and activator of transcription-3 maintains the stemness of radial glia at mid-neurogenesis. J Neurosci, 2015-01-21;35(3):1011-23. 2015-01-21 [PMID: 25609618] (Bioassay, Mouse)	Bioassay	Mouse
Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 2014-06-11;33(14):1582-98. 2014-06-11 [PMID: 24920579] (Bioassay, Mouse)	Bioassay	Mouse
Elsaeidi , Fairouz, Bemben , Michael, Zhao , Xiao-Fen, Goldman , Daniel Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci, 2014-02-12;34(7):2632-44. 2014-02-12 [PMID: 24523552] (Bioassay, Zebrafish)	Bioassay	Zebrafish
Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 2013-12-27;7(0):263. 2013-12-27 [PMID: 24409114] (In Vivo, Mouse)	In Vivo	Mouse
Dubovy P, Raska O, Klusakova I, Stejskal L, Celakovsky P, Haninec P Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy. BMC Neurosci, 2011-06-22;12(0):58. 2011-06-22 [PMID: 21696588] (In Vivo, Rat)	In Vivo	Rat
Krencik R, Weick JP, Liu Y, Zhang ZJ, Zhang SC Specification of transplantable astroglial subtypes from human pluripotent stem cells. Nat. Biotechnol., 2011-05-22;29(6):528-34. 2011-05-22 [PMID: 21602806] (Bioassay, Human)	Bioassay	Human
Kent TL, Glybina IV, Abrams GW, Iezzi R Chronic intravitreous infusion of ciliary neurotrophic factor modulates electrical retinal stimulation thresholds in the RCS rat. Invest. Ophthalmol. Vis. Sci., 2008-01-01;49(1):372-9. 2008-01-01 [PMID: 18172115] (In Vivo, Rat)	In Vivo	Rat
McGill TJ, Prusky GT, Douglas RM, Yasumura D, Matthes MT, Nune G, Donohue-Rolfe K, Yang H, Niculescu D, Hauswirth WW, Girman SV, Lund RD, Duncan JL, Lavail MM Intraocular CNTF reduces vision in normal rats in a dose-dependent manner. Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5756-66. 2007-12-01 [PMID: 18055829] (In Vivo, Rat)	In Vivo	Rat
Ko ML, Liu Y, Dryer SE, Ko GY The expression of L-type voltage-gated calcium channels in retinal photoreceptors is under circadian control. J. Neurochem., 2007-08-07;103(2):784-92. 2007-08-07 [PMID: 17683482] (Bioassay, Chicken)	Bioassay	Chicken
Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur. J. Neurosci., 2007-02-01;25(3):649-58. 2007-02-01 [PMID: 17328769] (Bioassay, Rat)	Bioassay	Rat
Janoschek R, Plum L, Koch L, Munzberg H, Diano S, Shanabrough M, Muller W, Horvath TL, Bruning JC gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor. Proc. Natl. Acad. Sci. U.S.A., 2006-07-03;103(28):10707-12. 2006-07-03 [PMID: 16818888] (In Vivo, Mouse)	In Vivo	Mouse
Hapner SJ, Nielsen KM, Chaverra M, Esper RM, Loeb JA, Lefcort F NT-3 and CNTF exert dose-dependent, pleiotropic effects on cells in the immature dorsal root ganglion: neuregulin-mediated proliferation of progenitor cells and neuronal differentiation. Dev. Biol., 2006-05-19;297(1):182-97. 2006-05-19 [PMID: 16784738] (Bioassay, Avian)	Bioassay	Avian
Murata T, Tsuboi M, Hikita K, Kaneda N Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J. Biol. Chem., 2006-06-13;281(32):22503-16. 2006-06-13 [PMID: 16772303] (Bioassay, Mouse)	Bioassay	Mouse
Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002. 2005-05-25 [PMID: 15917337] (Bioassay, Rat)	Bioassay	Rat
Fischer AJ, Omar G, Eubanks J, McGuire CR, Dierks BD, Reh TA Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage. Mol. Vis., 2004-12-22;10(0):973-86. 2004-12-22 [PMID: 15623987] (In Vivo, Chicken)	In Vivo	Chicken
Mizisin AP, Vu Y, Shuff M, Calcutt NA Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats. Diabetes, 2004-07-01;53(7):1807-12. 2004-07-01 [PMID: 15220205] (In Vivo, Rat)	In Vivo	Rat
Show All 36 Publications. Collapse Publications.

Array 557-NT

• CNTF Antibodies
• CNTF ELISA Kits
• CNTF Lysate
• CNTF Proteins
• CNTF Proteins and Enzymes
• CNTF ELISAs