CNTFR alpha Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha .

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Feline.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, Immunoprecipitation, Western Blot.

Showing Publications 1 - 8 of 8.

Publications using AF-303-NA	Applications	Species
Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J; Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR The Journal of biological chemistry 2024-04-01 [PMID: 38569939] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Min‐Tae Jeon, Gyeong Joon Moon, Sehwan Kim, Minji Choi, Yong‐Seok Oh, Dong Woon Kim et al. Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo British Journal of Pharmacology 2/1/2020 [PMID: 31658360]
Shay-Whey M. Koh, Timothy Coll, Dante Gloria, Nicholas Sprehe Corneal Endothelial Cell Integrity in Precut Human Donor Corneas Enhanced by Autocrine Vasoactive Intestinal Peptide Cornea 4/1/2017 [PMID: 28181929]
Shay-Whey M. Koh, Dante Gloria, Joseph Molloy Corneal Endothelial Autocrine VIP Enhances Its Integrity in Stored Human Donor Corneoscleral Explant Investigative Opthalmology & Visual Science 7/29/2011 [PMID: 21482640]
Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. Mol. Cell. Biol., 2010-06-28;17(0):4175. 2010-06-28 [PMID: 20584990] (Western Blot, Human)	Western Blot	Human
Yata T, Nakamura M, Sagawa H, Tokita Y, Terasaki H, Watanabe M Survival and axonal regeneration of off-center retinal ganglion cells of adult cats are promoted with an anti-glaucoma drug, nipradilol, but not BDNF and CNTF. Neuroscience, 2007-07-12;148(1):53-64. 2007-07-12 [PMID: 17629411] (IHC-Fr, Feline)	IHC-Fr	Feline
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005-08-15;579(20):4317-23. 2005-08-15 [PMID: 16051226] (Western Blot, Human)	Western Blot	Human
Koh SW Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo. Invest. Ophthalmol. Vis. Sci., 2002-09-01;43(9):2887-96. 2002-09-01 [PMID: 12202507] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IF	Mouse	12/19/2014	View Summary Application Immunofluorescence Sample Tested Mouse neurons DIV12 Species Mouse

Array AF-303-NA

• CNTFR alpha Antibodies
• CNTFR alpha Proteins and Enzymes