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Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind Recombinant Mouse IL‑33 (Catalog # 3626-ML) in a functional ELISA with an estimated KD < 50 nM. Schmitz, J. et al. (2005) Immunity 23:479.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ST2/IL-33R protein
Mouse ST2
(Ser27-Ser342)
Accession # P14719		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il1rl1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
63 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
73 kDa, reducing conditions
Publications
Read Publications using
1004-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

  • DER4
  • DER4ST2growth stimulation-expressed
  • Fit-1
  • IL-1 R4
  • IL1R4
  • IL-1R4
  • IL-1RL1
  • IL-33 R
  • IL33R
  • IL-33R
  • interleukin 1 receptor-like 1
  • interleukin 1 receptor-related protein
  • interleukin-1 receptor-like 1
  • Ly84
  • MGC32623
  • Protein ST2
  • ST2
  • ST2L
  • ST2V
  • T1
  • T1homolog of mouse growth stimulation-expressed

Background

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4-7). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11-13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12-14).

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Schmitz, J. et al. (2005) Immunity 23:479.
  5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
  9. Palmer, G. et al. (2008) Cytokine 42:358.
  10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

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Recombinant Mouse
ST2/IL-33R Fc Chimera ...
1004-MR
Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF
Species: Mu
Applications: Binding Activity

Publications for ST2/IL-33R (1004-MR)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 3 applications: Bioassay, ELISA (Capture), In Vivo.


Filter By Application
Bioassay
(5)
ELISA (Capture)
(1)
In Vivo
(3)
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Human
(1)
Mouse
(7)
Rabbit
(1)
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Showing Publications 1 - 9 of 9.
Publications using 1004-MR Applications Species
LD Faustino, JW Griffith, RA Rahimi, K Nepal, DL Hamilos, JL Cho, BD Medoff, JJ Moon, DAA Vignali, AD Luster Interleukin-33 activates regulatory T cells to suppress innate &amp;gamma&amp;delta T cell responses in the lung Nat Immunol, 2020-09-28;0(0):. 2020-09-28 [PMID: 32989331] (Bioassay, Mouse) Bioassay Mouse
AL Frisbee, MM Saleh, MK Young, JL Leslie, ME Simpson, MM Abhyankar, CA Cowardin, JZ Ma, P Pramoonjag, SD Turner, AP Liou, EL Buonomo, WA Petri IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection Nat Commun, 2019-06-20;10(1):2712. 2019-06-20 [PMID: 31221971] (In Vivo, Mouse) In Vivo Mouse
AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip IL-33 ameliorates Alzheimer&#039;s disease-like pathology and cognitive decline Proc Natl Acad Sci USA, 2016-04-18;0(0):. 2016-04-18 [PMID: 27091974] (In Vivo, Mouse) In Vivo Mouse
Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway. J Immunol, 2015-09-04;195(8):3537-45. 2015-09-04 [PMID: 26342029] (In Vivo, Mouse) In Vivo Mouse
Seeber S, Ros F, Thorey I, Tiefenthaler G, Kaluza K, Lifke V, Fischer J, Klostermann S, Endl J, Kopetzki E, Pashine A, Siewe B, Kaluza B, Platzer J, Offner S A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood. PLoS ONE, 2014-02-04;9(2):e86184. 2014-02-04 [PMID: 24503933] (Bioassay, Rabbit) Bioassay Rabbit
Williams , Jesse W, Tjota , Melissa, Clay , Bryan S, Vander Lugt , Bryan, Bandukwala , Hozefa S, Hrusch , Cara L, Decker , Donna C, Blaine , Kelly M, Fixsen , Bethany, Singh , Harinder, Sciammas , Roger, Sperling , Anne I Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun, 2013-01-01;4(0):2990. 2013-01-01 [PMID: 24356538] (Bioassay, Mouse) Bioassay Mouse
Drube S, Heink S, Walter S, Lohn T, Grusser M, Gerbaulet A, Berod L, Schons J, Dudeck A, Freitag J, Grotha S, Reich D, Rudeschko O, Norgauer J, Hartmann K, Roers A, Kamradt T The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells. Blood, 2010-03-03;115(19):3899-906. 2010-03-03 [PMID: 20200353] (Bioassay, Human) Bioassay Human
Ali S, Huber M, Kollewe C, Bischoff SC, Falk W, Martin MU IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells. Proc. Natl. Acad. Sci. U.S.A., 2007-11-14;104(47):18660-5. 2007-11-14 [PMID: 18003919] (Bioassay, Mouse) Bioassay Mouse
Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol., 2007-08-15;179(4):2551-5. 2007-08-15 [PMID: 17675517] (ELISA (Capture), Mouse) ELISA (Capture) Mouse

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Bioinformatics

Gene Symbol Il1rl1
Uniprot