Reactivity | MuSpecies Glossary |
Applications | Bioactivity |
Details of Functionality | Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 0.6-6 ng/mL. |
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Source | Mouse myeloma cell line, NS0-derived mouse IL-27 protein
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Accession # | |||||||||
N-terminal Sequence | Tyr19 |
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Protein/Peptide Type | Recombinant Proteins |
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Gene | Il27 |
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Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
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Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 48.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 60-65 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein. |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL-12), IL-27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N-linked glycosylation site and four alpha -helices, placing it in the IL-6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (7, 9, 10). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction (11).
Ly6G Antibody - A Marker for Monocytes, Granulocytes and Neutrophils What is Ly6G?Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. M... Read full blog post. |
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Gene Symbol | Il27 |
Uniprot |