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Recombinant Human VEGFR2/KDR His-tag Protein, CF

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Recombinant Human VEGFR2/KDR/Flk-1 His-tag Protein (Catalog # 10964-KD) inhibits the Recombinant Human VEGF165 (293-VE) dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.350-3.50 μg/mL.
Recombinant Human VEGFR2/KDR/Flk-1 His-tag Protein (Catalog # 10964-KD) inhibits the Recombinant Human VEGF165 (293-VE) dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect ...read more
2 μg/lane of Recombinant Human VEGFR2/KDR His-tag Protein (Catalog # 10964-KD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human VEGFR2/KDR His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.350-3.50 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human VEGFR2/KDR/Flk-1 protein
Ala20-Glu764, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala20
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
114-126 kDa

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1.00 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGFR2/KDR His-tag Protein, CF

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

Vascular endothelial Growth Factor Receptor 2 (VEGFR2), also known as FLK-1, KDR, and CD309, is a type I single-pass membrane receptor. Mature VEGFR2 contains a 745 amino acid extracelluar domain with seven immunoglobulin-like repeats, 21 transmembrane domain and 571 cytoplasmic domain. Within the extracellular domain, human VEGFR2 shares 80% homology with that of mouse and rat. VEGFR2, VEGFR1(Flt-1) and VEGFR3(Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors have almost exclusive expression in the endothelial cells and play essential roles in vasculogenesis and angiogenesis. VEGFR2 is the receptor for VEGF-A, VEGF-C, VEGF-D, and VEGF-E (viral homologs) (1, 2). Monomeric VEGFR2 dimerizes after binding to dimeric ligand and phosphorylate the Tyr in the cytoplasmic domain (3). VEGFR2 can also form heterodimer with VEGFR1 and VEGFR3 (4-6). Alternative splicing isoforms 2 and 3 which lack the transmembrane and cytoplasmic domains function as decoy receptors (7, 8). Targeting the signaling pathways of VEGFR1 and VEGFR2 are potential therapeutic targets for the treatment of inflammation and multiple tumors including breast, gastric, and lung carcinomas (9-12). Cancer immunotherapies using VEGF and VEGFR2 monoclonal antibodies may also be effective in combination with programmed cell death protein 1 (PD-1)/ programmed cell death ligand 1 (PD-L1) immune checkpoint blockade (13). 
  1. Ferra, N. and Davis-Smyth, T. (1997) Endocr. Rev. 18:4.
  2. Wise, L.M. et al. (2012) Cell Microbiol. 13:1376.
  3. Lepanen, V.M. et al. (2010) Proc. Natl. Acad. Sci. USA 107:2425.
  4. Cai, M. et al. (2017) Vascul. Pharmacol. 88:11.
  5. Nilsson, I. et al. (2010) EMBO J. 29:1377.
  6. Dixelius, J. et al. (2003) J. Biol. Chem. 278:40973.
  7. Albuquerque, R.J. et al. (2009) Nat. Med. 15:1023.
  8. Jin, P. et al. (2008) Arthritis Res. Ther. 10:R73.
  9. Shibuya, M. (2015) Endocr Metab Immune Disord Drug Targets. 15:135.
  10. Farzaneh Behelgardi, M. et al. (2020), Mol Biol Rep. 47:2061.
  11. Lian, L. et al. (2019) BMC Cancer. 19:183.
  12. Hu, C. et al. (2019) Onco. Targets Ther. 12:933.
  13. Gao, F. et al. (2020) Curr. Cancer Drug Targets. 20:3.

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Recombinant Human
VEGFR2/KDR His-tag Pr ...
10964-KD
Recombinant Human VEGFR2/KDR His-tag Protein, CF
Species: Hu
Applications: Bioactivity

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