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Recombinant Human TSLPR His-tag Avi-tag Protein, CF

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2 μg/lane of Biotinylated Recombinant Human TSLPR His-tag Avi-tag Protein (Catalog # AVI10843) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TSLPR His-tag Avi-tag Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human TSLP (Catalog # 1398-TS) is immobilized at 0.500 μg/mL (100 μL/well), Biotinylated Recombinant Human TSLP His-tag Avi-tag (Catalog # AVI10841) binds with an ED50 of 0.100-0.800  μg/mL. The biotin to protein ratio is greater than 0.7 as deeterminded by the HABA assay.
Source
Chinese Hamster Ovary cell line, CHO-derived human TSLPR protein
Human TSLPR
(Gly25-Lys231)
Accession # Q9HC73.1
6-His tagAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Gly25 and Val30
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
26 kDa and 27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-55 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSLPR His-tag Avi-tag Protein, CF

  • CRL2
  • CRL2cytokine receptor CRL2 precusor
  • CRLF2
  • CRLF2Y
  • CRLM-2
  • Cytokine receptor-like 2
  • cytokine receptor-like factor 2
  • ILXR
  • IL-XR
  • P2RY8/CRLF2 fusion
  • PCOR1
  • Thymic stromal lymphopoietin protein receptor
  • thymic stromal lymphopoietin receptor
  • thymic stromal-derived lymphopoietin receptor
  • TSLP R
  • TSLP receptor
  • TSLPR

Background

Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like module 2 (CRLM-2) and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS‑like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (2, 3). The ECD of Human TSLPR shares 34% amino acid sequence identity with the ECD of mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up-regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4). Our Avi-tag Biotinylated Recombinant Human TSLPR protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

  1. Park LS et al. (2000) J. Exp. Med. 192:659.
  2. Blagoev, B. et al. (2002) Gene. 284:161.
  3. Ziegler, S.F. et al. (2013) Advances in Pharmacology. 66:129.
  4. He, R. et al. (2010) Ann N Y Acad Sci. 1183:13.
  5. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  6. Headley, M.B. et al. (2009) J Imunol. 182:1641.
  7. Soumelis, V. et al. (2002) Nat Immunol. 3:673.

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