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Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
372-RI-050/CF
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Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein Summary

Details of Functionality
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.4‑2 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA).
Source
Mouse myeloma cell line, NS0-derived human TNF RI/TNFRSF1A protein
Human TNF RI
(Leu30-Thr211) &
(Asp41-Thr211)
Accession # P19438		 IEGRMD Human IgG1
(Pro100-Lys330)		 6 His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu30 & Asp41
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TNFRSF1A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
48 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-66 kDa, reducing conditions
Publications
Read Publications using
372-RI in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence and 190 aa ECD with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of human TNF RI shows 70%, 69%, 80%, 80%, and 73% aa identity with mouse, rat, canine, feline and porcine TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Schall, T.J. et al. (1990) Cell 61:361.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.

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Publications for TNF RI/TNFRSF1A (372-RI)(11)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria, N/A.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, PcIP.


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Bioassay
(8)
Cell Culture
(2)
In Vivo
(1)
PcIP
(1)
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Human
(6)
Mouse
(1)
Rat
(1)
Bacteria
(1)
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(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 372-RI Applications Species
Gesiorowski, A;Ettich, J;Werner, J;Wittich, C;Pieper, S;Padrini, G;Behnke, K;Floss, DM;Lang, PA;Moll, JM;Scheller, J; Bispecific soluble cytokine receptor-nanobody fusions inhibit Interleukin (IL-)6 trans-signaling and IL-12/23 or tumor necrosis factor (TNF) signaling The Journal of biological chemistry 2023-10-12 [PMID: 37838173] (Bioassay, N/A) Bioassay N/A
DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF Nature Communications, 2021-01-25;12(1):583. 2021-01-25 [PMID: 33495445] (Bioassay, Rat) Bioassay Rat
T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh Defective Regulation of Membrane TNF&amp;alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells Int J Mol Sci, 2020-04-21;21(8):. 2020-04-21 [PMID: 32326230] (Cell Culture, Human) Cell Culture Human
M Inoue, D Ando, H Kamada, S Taki, M Niiyama, Y Mukai, T Tadokoro, K Maenaka, T Nakayama, Y Kado, T Inoue, Y Tsutsumi, SI Tsunoda A trimeric structural fusion of an antagonistic tumor necrosis factor-? mutant enhances molecular stability and enables facile modification J. Biol. Chem, 2017-02-24;0(0):. 2017-02-24 [PMID: 28235800] (Bioassay) Bioassay
Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells. J Biol Chem, 2014-02-18;289(14):10029-44. 2014-02-18 [PMID: 24550382] (Bioassay, Cell Culture, Human) Bioassay, Cell Culture Human
Moh M, Lorenzini P, Gullo C, Schwarz H Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J, 2013-04-25;27(8):2957-66. 2013-04-25 [PMID: 23620528] (Bioassay, Human) Bioassay Human
Cosgrove C, Ussher J, Rauch A, Gartner K, Kurioka A, Huhn M, Adelmann K, Kang Y, Fergusson J, Simmonds P, Goulder P, Hansen T, Fox J, Gunthard H, Khanna N, Powrie F, Steel A, Gazzard B, Phillips R, Frater J, Uhlig H, Klenerman P Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection. Blood, 2012-12-18;121(6):951-61. 2012-12-18 [PMID: 23255555] (Bioassay, Human) Bioassay Human
Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-06-09;76(8):3530-8. 2008-06-09 [PMID: 18541656] (PcIP, Bacteria) PcIP Bacteria
Narita M, Shimamura M, Imai S, Kubota C, Yajima Y, Takagi T, Shiokawa M, Inoue T, Suzuki M, Suzuki T Role of interleukin-1beta and tumor necrosis factor-alpha-dependent expression of cyclooxygenase-2 mRNA in thermal hyperalgesia induced by chronic inflammation in mice. Neuroscience, 2007-11-12;152(2):477-86. 2007-11-12 [PMID: 18262365] (In Vivo, Mouse) In Vivo Mouse
Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression. Mol. Cell. Biol., 2006-04-01;26(8):3071-84. 2006-04-01 [PMID: 16581782] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol TNFRSF1A
Uniprot