Home » ICAM-3/CD50 » ICAM-3/CD50 Proteins » Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

 (3 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for ICAM-3/CD50 (715-IC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
715-IC-200
Novus Biologicals offers 100% Guarantee

Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 40-70% will adhere after PMA stimulation for 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-3/CD50 protein
Human ICAM-3
(Gln30 - His485)
Accession # CAA49473		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln30 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ICAM3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
76.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
715-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Customers Who Viewed This Item Also Viewed...

HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Integrin beta
2/CD18 Antibody [Unco ...
AF1730
Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]
Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]
Flow Cytometry Integrin beta 2/CD18 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
Integrin alpha
L/CD11a Antibody (MEM ...
NB500-328
Flow Cytometry Integrin alpha L/CD11a Antibody (MEM-25) - BSA Free
Flow Cytometry Integrin alpha L/CD11a Antibody (MEM-25) - BSA Free
Flow Cytometry Integrin alpha L/CD11a Antibody (MEM-25) - BSA Free
(10 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IP
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(89 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
ICAM-2/CD102
Antibody [Unconjugate
AF244
Western Blot ICAM-2/CD102 Antibody [Unconjugated]
Western Blot ICAM-2/CD102 Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, WB
DC-SIGN/CD209
Antibody (120507) [Un ...
MAB161
Flow Cytometry DC-SIGN/CD209 Antibody (120507) [Unconjugated]
Flow Cytometry DC-SIGN/CD209 Antibody (120507) [Unconjugated]
Flow Cytometry DC-SIGN/CD209 Antibody (120507) [Unconjugated]
(1 Review)
(48 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, WB
Lymphotoxin beta
R/TNFRSF3 Antibody
AF629
Lymphotoxin beta R/TNFRSF3 Antibody
(10 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, InhibCellGro, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(822 Publications)
Species: Hu
Applications: BA
CD11b Antibody -
BSA Free
NB110-89474
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
(12 Reviews)
(128 Publications)
Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
Applications: Dual ISH-IHC, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB

DC-SIGNR/CD299/CLEC4M ...
MAB162
Flow Cytometry DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]
Immunocytochemistry/ Immunofluorescence DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]
Immunocytochemistry/ Immunofluorescence DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]
(18 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow
E-Selectin/CD62E
Antibody (BBIG-E4) [U ...
BBA16
Immunohistochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
Immunocytochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
Flow Cytometry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
(1 Review)
(60 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
L-Selectin/CD62L
Antibody (4G8) [Uncon ...
BBA24
Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]
(9 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow
CD31/PECAM-1
Antibody [Unconjugate
AF3628
Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]
Flow Cytometry CD31/PECAM-1 Antibody [Unconjugated]
Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]
(6 Reviews)
(513 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(52 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
B7-1/CD80 Antibody
(37711) [Unconjugated ...
MAB140
Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]
Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]
Dual RNAscope ISH-IHC Compatible B7-1/CD80 Antibody (37711) [Unconjugated]
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut

Publications for ICAM-3/CD50 (715-IC)(3)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(2)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species

Reviews for ICAM-3/CD50 (715-IC) (0)

There are no reviews for ICAM-3/CD50 (715-IC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ICAM-3/CD50 (715-IC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ICAM-3/CD50 Products

Blogs on ICAM-3/CD50

There are no specific blogs for ICAM-3/CD50, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
CD31/PECAM-1 Antibody [Unconjugated]
AF3628
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol ICAM3
Uniprot