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Recombinant Human ACE-2 Protein, CF

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Recombinant Human ACE-2/His (Catalog # 933-ZN) has a molecular weight (MW) of 189.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational ...read more
Four independent lots of recombinant Human ACE-2 His-tag 933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph ...read more
Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 ...read more
Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).
1 μg/lane of Recombinant Human ACE-2 Protein (Catalog # 933-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at ~106 kDa under reducing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity, Enzyme Activity
Format
Carrier-Free

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Catalog# & Formulation Size Price
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Recombinant Human ACE-2 Protein, CF Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds with an ED50 of 1.00-12.0 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained: Gln18 predicted
Structure / Form
Recombinant Human ACE‑2 is prone to proteolytic cleavage at C-terminus. 
Protein/Peptide Type
Recombinant Enzymes
Gene
ACE2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity2
  • Enzyme Activity
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
101-111 kDa, reducing conditions
Publications
Read Publications using
933-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ACE-2 Protein, CF

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12).  Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

  1. Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
  2. Yan, et al. (2020) Science 367:1444.
  3. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Hoffmann, M. et al. (2020) Cell.181:1.
  6. Wrapp, et al. (2020) Science 367:1260.
  7. Imai, Y. et al. (2005) Nature 436:112.
  8. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  9. Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
  10. Jia, H. et al. (2016) Shock. 46:239.
  11. Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
  12. Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
  13. Crackower, M.A. et al. (2002) Nature 417:822.

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Publications for ACE-2 (933-ZN)(27)

We have publications tested in 6 confirmed species: Human, Mouse, Canine, Feline, Primate - Chlorocebus pygerythrus (Vervet Monkey), Virus.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Enzyme Assay, Flow Cytometry, In Vivo, Reference Standard, Surface Plasmon Resonance (SPR, Western Blot Standard.


Filter By Application
Binding Assay
(1)
Bioassay
(18)
Cell Culture
(1)
Enzyme Assay
(3)
Flow Cytometry
(1)
In Vivo
(1)
Reference Standard
(1)
Surface Plasmon Resonance (SPR
(1)
Western Blot Standard
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(1)
Canine
(2)
Feline
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 933-ZN Applications Species
Gaglione, SA;Rosales, TJ;Schmidt-Hong, L;Baker, BM;Birnbaum, ME; SARS-CoV-2 spike does not interact with the T cell receptor or directly activate T cells Proceedings of the National Academy of Sciences of the United States of America 2024-07-30 [PMID: 39042676] (Bioassay, Human) Bioassay Human
Bhargavan, B;Kanmogne, GD; SARS-CoV-2 Spike Proteins and Cell-Cell Communication Induce P-Selectin and Markers of Endothelial Injury, NETosis, and Inflammation in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for the Pathogenesis of COVID-19 Coagulopathy International journal of molecular sciences 2023-08-09 [PMID: 37628764] (Bioassay, Human) Bioassay Human
Bouillet, L;Deroux, A;Benmarce, M;Guérin, C;Bouvet, L;Garnier, O;Martin, DK;Vilgrain, I; Molecular Mechanisms of Endothelialitis in SARS-CoV-2 Infection: Evidence for VE-Cadherin Cleavage by ACE2 International journal of molecular sciences 2023-08-07 [PMID: 37569899] (Western Blot Standard, Human) Western Blot Standard Human
Suresh, V;Sheik, DA;Detomasi, TC;Zhao, T;Zepeda, T;Saladi, S;Rajesh, UC;Byers, K;Craik, CS;Davisson, VJ; A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19 Biosensors 2023-06-27 [PMID: 37504081] (Bioassay) Bioassay
L Fu, D Gilham, SC Stotz, CD Sarsons, BD Rakai, LM Tsujikawa, S Wasiak, JO Johansson, M Sweeney, NCW Wong, E Kulikowski Dual mechanism: Epigenetic inhibitor apabetalone reduces SARS-CoV-2 Delta and Omicron variant spike binding and attenuates SARS-CoV-2 RNA induced inflammation International immunopharmacology, 2023-02-23;117(0):109929. 2023-02-23 [PMID: 36857935] (Bioassay, Human) Bioassay Human
X Guo, J Cao, JP Cai, J Wu, J Huang, P Asthana, SKK Wong, ZW Ye, S Gurung, Y Zhang, S Wang, Z Wang, X Ge, HY Kwan, A Lyu, KM Chan, N Wong, J Huang, Z Zhou, ZX Bian, S Yuan, HLX Wong Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2 Nature Communications, 2022-12-23;13(1):7907. 2022-12-23 [PMID: 36564389] (Bioassay, Human) Bioassay Human
JW Kim, SW Min, J Lee, HG Shin, HL Choi, HR Yang, JH Lee, YB Cho, H Shim, S Lee Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2 Biomedicines, 2022-12-17;10(12):. 2022-12-17 [PMID: 36552031] (Bioassay, Human) Bioassay Human
R Reindl-Sch, S Hödlmoser, O Domenig, K Krenn, F Eskandary, S Krenn, C Schörgenho, B Rumpf, M Karolyi, MT Traugott, A Abrahamowi, V Tinhof, H Mayfurth, V Rathkolb, S Mu beta nig, L Schmölz, R Ullrich, A Heinzel, F König, C Binder, D Bonderman, R Strassl, E Puchhammer, G Gorkiewicz, JH Aberle, B Jilma, C Wenisch, M Poglitsch, R Oberbauer, A Zoufaly, M Hecking The systemic renin-angiotensin system in COVID-19 Scientific Reports, 2022-11-22;12(1):20117. 2022-11-22 [PMID: 36418458] (Bioassay, Human) Bioassay Human
B Bhargavan, GD Kanmogne SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis International Journal of Molecular Sciences, 2022-09-09;23(18):. 2022-09-09 [PMID: 36142345] (Bioassay, Human) Bioassay Human
M Haddad, R Gaudreault, G Sasseville, PT Nguyen, H Wiebe, T Van De Ven, S Bourgault, N Mousseau, C Ramassamy Molecular Interactions of Tannic Acid with Proteins Associated with SARS-CoV-2 Infectivity International Journal of Molecular Sciences, 2022-02-27;23(5):. 2022-02-27 [PMID: 35269785] (Bioassay) Bioassay
Show All 27 Publications.

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Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Bioinformatics

Gene Symbol ACE2
Uniprot