Hi, I have two questions about MAS1 Antibody (NBP1-78444). Could you tell me if it could detect N-terminal tag3* flag-Mas or not? The human N-terminal tag3* flag-Mas vector that I constructed were veri?ed by DNA sequencing and were consistent with the NCBI reference sequence: NM_002377.2. It's open reading frame-shift did not appear and MAS1 is about 34KD. Why is the MW of MAS1 is bigger than 37KD on your datasheet?

Name: Mas Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-78444
Rating: 5 (2 reviews)

Question

Accepted Answer

I have pulled up information on your NCBI sequence and it matches that associated with the uniprot ID P04201 for this antibody (https://www.uniprot.org/uniprotkb/P04201/entry) The protein appears to theoretically have a molecular weight of 37 kDa, but most likely due to post translational modifications such as glycosylation we expressed the protein at a higher molecular weight in our sample, which can be common for proteins that under go post translational modifications such as this one. We will back this antibody with our 100% guarantee for all stated species and applications.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications	WB, Simple Western, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-78444AF350

Alexa Fluor 405 NBP1-78444AF405

Alexa Fluor 488 NBP1-78444AF488

Alexa Fluor 532 NBP1-78444AF532

Alexa Fluor 594 NBP1-78444AF594

Alexa Fluor 647 NBP1-78444AF647

Alexa Fluor 700 NBP1-78444AF700

Alexa Fluor 750 NBP1-78444AF750

Allophycocyanin NBP1-78444APC

Biotin NBP1-78444B

CoraFluor 1 NBP1-78444CL1

DyLight 350 NBP1-78444UV

DyLight 405 NBP1-78444V

DyLight 488 NBP1-78444G

DyLight 550 NBP1-78444R

DyLight 594 NBP1-78444DL594

DyLight 650 NBP1-78444C

DyLight 680 NBP1-78444FR

DyLight 755 NBP1-78444IR

FITC NBP1-78444F

HRP NBP1-78444H

Janelia Fluor 525 NBP1-78444JF525

Janelia Fluor 549 NBP1-78444JF549

Janelia Fluor 585 NBP1-78444JF585

Janelia Fluor 635 NBP1-78444JF635

Janelia Fluor 646 NBP1-78444JF646

Janelia Fluor 669 NBP1-78444JF669

PE NBP1-78444PE

PerCP NBP1-78444PCP

mFluor Violet 450 SE NBP1-78444MFV450

mFluor Violet 500 SE NBP1-78444MFV500

mFluor Violet 610 SE NBP1-78444MFV610

Catalog# & Formulation	Size	Price
BSA Free NBP1-78444SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78444AF350
Alexa Fluor 405	NBP1-78444AF405
Alexa Fluor 488	NBP1-78444AF488
Alexa Fluor 532	NBP1-78444AF532
Alexa Fluor 594	NBP1-78444AF594
Alexa Fluor 647	NBP1-78444AF647
Alexa Fluor 700	NBP1-78444AF700
Alexa Fluor 750	NBP1-78444AF750
Allophycocyanin	NBP1-78444APC
Biotin	NBP1-78444B
CoraFluor 1	NBP1-78444CL1
DyLight 350	NBP1-78444UV
DyLight 405	NBP1-78444V
DyLight 488	NBP1-78444G
DyLight 550	NBP1-78444R
DyLight 594	NBP1-78444DL594
DyLight 650	NBP1-78444C
DyLight 680	NBP1-78444FR
DyLight 755	NBP1-78444IR
FITC	NBP1-78444F
HRP	NBP1-78444H
Janelia Fluor 525	NBP1-78444JF525
Janelia Fluor 549	NBP1-78444JF549
Janelia Fluor 585	NBP1-78444JF585
Janelia Fluor 635	NBP1-78444JF635
Janelia Fluor 646	NBP1-78444JF646
Janelia Fluor 669	NBP1-78444JF669
PE	NBP1-78444PE
PerCP	NBP1-78444PCP
mFluor Violet 450 SE	NBP1-78444MFV450
mFluor Violet 500 SE	NBP1-78444MFV500
mFluor Violet 610 SE	NBP1-78444MFV610

Mas Antibody - BSA Free

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Publications for Mas Antibody (NBP1-78444)(21)

Reviews for Mas Antibody (NBP1-78444) (2) 52

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Immunogen	A synthetic peptide made to an internal portion of the human MAS1 protein (between residues 75-125) [Uniprot: P04201]
Localization	Cell membrane
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MAS1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS and 30% Glycerol
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Immunogen affinity purified

Publications using NBP1-78444	Applications	Species
Fan H, Wu K, Wu J LRW fails to reduce blood pressure in spontaneously hypertensive rats due to its low gastrointestinal stability and transepithelial permeability Food Bioscience 2022-08-01 (WB, Rat)	WB	Rat
Zhu C, Gu W, Sun D, Wei W The mechanism underlying fluoride-induced low-renin hypertension is related to an imbalance in the circulatory and local renin-angiotensin systems Toxicology letters 2023-04-25 [PMID: 37105417] (WB, Rat)	WB	Rat
Juretzko A, Steinbach A, Witte J et al. Renal angiotensin I-converting enzyme-deficient mice are protected against aristolochic acid nephropathy Pflugers Archiv : European journal of physiology 2022-12-15 [PMID: 36520238] (WB, Mouse)	WB	Mouse
Matsunobe M, Motohashi N, Aoki E et al. Caveolin-3 regulates the activity of Ca2+/calmodulin-dependent protein kinase II in C2C12 cells American journal of physiology. Cell physiology 2022-08-22 [PMID: 35993515]
Lamy GB, Cafarchio EM, do Vale B et al. Unveiling the Angiotensin-(1-7) Actions on the Urinary Bladder in Female Rats Frontiers in Physiology 2022-07-19 [PMID: 35928558] (IP, WB)	IP, WB
Le Nguyen B, Yoshihara T, Deminice R et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers Sports Med Health Sci 2022-07-05 [PMID: 35784524]
Gao Q, Chen R, Wu L et al. Angiotensin-(1-7) reduces alpha -synuclein aggregation by enhancing autophagic activity in Parkinson's disease Neural regeneration research 2022-05-01 [PMID: 34558543] (WB, Rat)	WB	Rat
Dang R, Yang M, Cui C et al. Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling Aging cell 2021-09-16 [PMID: 34529881] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Lamy Gb, Cafarchio Em, Do Vale B Et Al. Lateral Preoptic Area Neurons Activated by Angiotensin-(1-7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control Frontiers in physiology 2021-07-12 [PMID: 34322035]
Yoshihara T, Deminice R, Hyatt H et al. Angiotensin 1-7 protects against ventilator-induced diaphragm dysfunction Clinical and translational science 2021-03-20 [PMID: 33742769] (WB, Rat)	WB	Rat
Fan H, Bhullar K, Wu J Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells Antioxidants 2021-02-15 [PMID: 33671990] (WB, Human, Rat)	WB	Human, Rat
Dang Z, Su S, Jin G, et al. Tsantan Sumtang attenuated chronic hypoxia-induced right ventricular structure remodeling and fibrosis by equilibrating local ACE-AngII-AT1R/ACE2-Ang1-7-Mas axis in rat J Ethnopharmacol 2019-12-17 [PMID: 31862407] (IF/IHC, Rat)	IF/IHC	Rat
Stone RE, Liu S, Levy AM, et al. Activation of the Protective Arm of the Renin Angiotensin System in Demyelinating Disease J Neuroimmune Pharmacol 2019-12-11 [PMID: 31828731]
Cerniello FM, Silva MG, Carretero OA, Gironacci MM Mas receptor is translocated to the nucleus upon agonist stimulation in brainstem neurons from spontaneously hypertensive rats but not normotensive rats Cardiovasc. Res. 2019-12-11 [PMID: 31825460] (WB, Rat)	WB	Rat
Cui C, Xu P, Li G et al. Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system Redox Biol 2019-08-08 [PMID: 31421410] (WB, Mouse)	WB	Mouse
Mo J, Enkhjargal B, Travis ZD et al. AVE 0991 attenuates oxidative stress and neuronal apoptosis via Mas/PKA/CREB/UCP-2 pathway after subarachnoid hemorrhage in rats Redox Biol. 2018-09-27 [PMID: 30296700] (WB, ICC/IF, Rat)	WB, ICC/IF	Rat
Ma X, Pang Z, Zhou J et al. Acetylation and Amination Protect Angiotensin 1-7 from Physiological Hydrolyzation and Therefore Increases Its Antitumor Effects on Lung Cancer Mol. Pharm. 2018-04-27 [PMID: 29685037] (WB, Mouse)	WB	Mouse
Zhao S, Ghosh A, Lo CS et al. Nrf2 Deficiency Upregulates Intrarenal Angiotensin-converting Enzyme-2 and Angiotensin 1-7 Receptor Expression and Attenuates Hypertension and Nephropathy in Diabetic Mice Endocrinology [PMID: 29211853] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Wheway G, Schmidts M, Mans DA et al. An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat Cell Biol 2015-08-01 [PMID: 26167768] (ICC/IF, Human)	ICC/IF	Human
Bian W, Sun L, Yang L et al. Stabilization of the Angiotensin-(1-7) Receptor Mas through Interaction with PSD95. Biochem J 2013-05-24 [PMID: 23701246] (WB, Rabbit)	WB	Rabbit
da Silveira KD, Coelho FM, Vieira AT et al. Anti-inflammatory effects of the activation of the angiotensin-(1-7) receptor, MAS, in experimental models of arthritis J Immunol 2010-11-01 [PMID: 20935211] (WB, Mouse)	WB	Mouse
Show All 21 Publications. Collapse Publications.

Gene Symbol	MAS1
Entrez	4142(Human)
Uniprot	P04201(Human)

	Kasia Szymanska 10/30/2013
Application:	WB
Species:	Human

	Gabrielle Wheway 10/14/2013
Application:	IF
Species:	Mouse