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Mas Antibody - BSA Free

Images

 
Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.
Immunocytochemistry/ Immunofluorescence: Mas Antibody [NBP1-78444] - Tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: Mas Antibody [NBP1-78444] - Lane view shows a specific band for MAS1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Mas Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human MAS1 protein (between residues 75-125) [Uniprot: P04201]
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:25
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-78444 in the following applications:

Publications
Read Publications using
NBP1-78444 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34558543). Rabbit reactivity reported in the scientific literature (PMID: 23701246).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mas Antibody - BSA Free

  • Mas
  • MAS1 oncogene
  • MAS1
  • MGC119966
  • proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-78444
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC

Publications for Mas Antibody (NBP1-78444)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-P, Immunoprecipitation, WB, Western Blot.


Filter By Application
ICC/IF
(2)
IF/IHC
(2)
IHC-P
(1)
Immunoprecipitation
(1)
WB
(14)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
Rat
(8)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP1-78444 Applications Species
Fan H, Wu K, Wu J LRW fails to reduce blood pressure in spontaneously hypertensive rats due to its low gastrointestinal stability and transepithelial permeability Food Bioscience 2022-08-01 (WB, Rat) WB Rat
Zhu C, Gu W, Sun D, Wei W The mechanism underlying fluoride-induced low-renin hypertension is related to an imbalance in the circulatory and local renin-angiotensin systems Toxicology letters 2023-04-25 [PMID: 37105417] (WB, Rat) WB Rat
Juretzko A, Steinbach A, Witte J et al. Renal angiotensin I-converting enzyme-deficient mice are protected against aristolochic acid nephropathy Pflugers Archiv : European journal of physiology 2022-12-15 [PMID: 36520238] (WB, Mouse) WB Mouse
Matsunobe M, Motohashi N, Aoki E et al. Caveolin-3 regulates the activity of Ca2+/calmodulin-dependent protein kinase II in C2C12 cells American journal of physiology. Cell physiology 2022-08-22 [PMID: 35993515]
Lamy GB, Cafarchio EM, do Vale B et al. Unveiling the Angiotensin-(1-7) Actions on the Urinary Bladder in Female Rats Frontiers in Physiology 2022-07-19 [PMID: 35928558] (Immunoprecipitation, Western Blot) Immunoprecipitation, Western Blot
Le Nguyen B, Yoshihara T, Deminice R et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers Sports Med Health Sci 2022-07-05 [PMID: 35784524]
Gao Q, Chen R, Wu L et al. Angiotensin-(1-7) reduces alpha -synuclein aggregation by enhancing autophagic activity in Parkinson's disease Neural regeneration research 2022-05-01 [PMID: 34558543] (WB, Rat) WB Rat
Dang R, Yang M, Cui C et al. Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling Aging cell 2021-09-16 [PMID: 34529881] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Lamy Gb, Cafarchio Em, Do Vale B Et Al. Lateral Preoptic Area Neurons Activated by Angiotensin-(1-7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control Frontiers in physiology 2021-07-12 [PMID: 34322035]
Yoshihara T, Deminice R, Hyatt H et al. Angiotensin 1-7 protects against ventilator-induced diaphragm dysfunction Clinical and translational science 2021-03-20 [PMID: 33742769] (WB, Rat) WB Rat
Show All 21 Publications.

Reviews for Mas Antibody (NBP1-78444) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-78444:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Mas NBP1-78444
Enlarge
5
reviewed by:
Kasia Szymanska
WB Human 10/30/2013
View

Summary

ApplicationWestern Blot
Sample TestedIMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
SpeciesHuman
LotA-1
Immunofluorescence Mas NBP1-78444
Enlarge
5
reviewed by:
Gabrielle Wheway
IF Mouse 10/14/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse inner medullary collecting duct (IMCD3) cell line
SpeciesMouse
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mas Antibody (NBP1-78444). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I have two questions about MAS1 Antibody (NBP1-78444). Could you tell me if it could detect N-terminal tag3* flag-Mas or not? The human N-terminal tag3* flag-Mas vector that I constructed were veri?ed by DNA sequencing and were consistent with the NCBI reference sequence: NM_002377.2. It's open reading frame-shift did not appear and MAS1 is about 34KD. Why is the MW of MAS1 is bigger than 37KD on your datasheet?
    • I have pulled up information on your NCBI sequence and it matches that associated with the uniprot ID P04201 for this antibody (https://www.uniprot.org/uniprotkb/P04201/entry) The protein appears to theoretically have a molecular weight of 37 kDa, but most likely due to post translational modifications such as glycosylation we expressed the protein at a higher molecular weight in our sample, which can be common for proteins that under go post translational modifications such as this one. We will back this antibody with our 100% guarantee for all stated species and applications.

Secondary Antibodies

 

Isotype Controls

Additional Mas Products

Array NBP1-78444

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Kasia Szymanska
10/30/2013
Application: WB
Species: Human

Gabrielle Wheway
10/14/2013
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol MAS1
Entrez
Uniprot