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RBBP4/RbAp48 Antibody (15G12)

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Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - RBBP4/RbAp48 antibody [15G12] detects RBBP4/RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RBBP4/RbAp48 stained by RBBP4/RbAp48 antibody [15G12] (NBP1-41202) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Scale bar= 10um.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - RBBP4/RbAp48 antibody [15G12] detects RBBP4/RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ...read more
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - RbAp48 + RbAp46 antibody detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical ...read more
Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Analysis of paraffin-embedded H1299 xenograft, using RbAp48 antibody at 1:200 dilution.
Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Analysis of paraffin-embedded C2C12 xenograft, using RbAp48 antibody at 1:200 dilution.
Western Blot: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RBBP4/RbAp48 antibody [15G12] (NBP1-41202) diluted at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
15G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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RBBP4/RbAp48 Antibody (15G12) Summary

Immunogen
The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Localization
Nuclear
Specificity
This antibody will recognize human RbAp48 + RbAp46.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RBBP4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 0.2-1 ug/ml
  • Western Blot 1:500-1:3000
Application Notes
EMSA. WB: Predicted MW is 47.6 kDa.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RBBP4/RbAp48 Antibody (15G12)

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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RBBP4/RbAp48
Antibody (15G12)
NBP1-41202
RBBP4/RbAp48 Antibody (15G12)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IP

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Bioinformatics

Gene Symbol RBBP4
Entrez