![Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-11G10-Western-Blot-NB500-123-img0011.jpg "Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC, IP, B/N, ChIP |
| Clone | 11G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli. |
| Specificity | This is specific for full length RbAp48. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RBBP4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF: Permeabilization step recommended. |
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| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Robert Lu |
IP | Human | 12/18/2020 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for RBBP4/RbAp48 Antibody (NB500-123)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RbAp48-Antibody-1D11-Immunocytochemistry-Immunofluorescence-NB500-123-img0003.jpg "Immunocytochemistry/Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.")
![Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]](http://images.novusbio.com/fullsize/RbAp48-Antibody-1D11-Immunohistochemistry-NB500-123-img0001.jpg "Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]")
![Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-11G10-Chromatin-Immunoprecipitation-NB500-123-img0007.jpg "Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.")
![Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-11G10-Western-Blot-NB500-123-img0009.jpg "Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-11G10-Immunoprecipitation-NB500-123-img0012.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. Image submitted by a verified customer review.")
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