![Western Blot: Ras Antibody (JE44-13) [NBP3-33138] - Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (NBP3-33138) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: A375 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33138) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33138_rabbit-ras-mab-je44-13-28820241423314.jpg "Western Blot: Ras Antibody (JE44-13) [NBP3-33138] - Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (NBP3-33138) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: A375 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33138) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | JE44-13 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human Ras aa 1-50 / 189. (Uniprot: P01111) |
| Localization | Cell membrane, Golgi apparatus membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/ Immunofluorescence: Ras Antibody (JE44-13) [NBP3-33138] - Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33138_rabbit-ras-mab-je44-13-29202410334099.jpg "Immunocytochemistry/ Immunofluorescence: Ras Antibody (JE44-13) [NBP3-33138] - Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
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