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Pyridoxal Kinase/PDXK Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding PDXK RNA-seq data are presented for ...read more
Independent Antibodies: Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Analysis using Anti-PDXK antibody NBP1-88284 (A) shows similar pattern to independent antibody NBP1-88283 (B).
Immunocytochemistry/ Immunofluorescence: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Staining of human skeletal muscle shows very weak positivity in a subset of myocytes.
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88284] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Pyridoxal Kinase/PDXK Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDXK
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Pyridoxal Kinase/PDXK Protein (NBP1-88284PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pyridoxal Kinase/PDXK Antibody

  • C21orf124
  • C21orf97
  • chromosome 21 open reading frame 124
  • DKFZp566A071
  • EC 2.7.1.35
  • FLJ21324
  • FLJ37311
  • HEL-S-1a
  • MGC15873
  • MGC31754
  • MGC52346
  • PDXK
  • PKH
  • PKHchromosome 21 open reading frame 97
  • PNK
  • PNKhuman pyridoxal kinase, EC 2.7.1.3510FLJ31940
  • PRED79
  • pyridoxal (pyridoxine, vitamin B6) kinase
  • Pyridoxal Kinase
  • pyridoxamine kinase
  • Pyridoxine kinase
  • Vitamin B6 Kinase

Background

Pyridoxal Kinase/PDXK is encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PDXK