PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354)

Images

 
Western Blot: PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354) [NBP3-07576] - Western Blot Analysis of human LNCaP cell lysate using PSMA/FOLH1/NAALADase I Mouse Monoclonal Antibody (FOLH1/2354).
Protein Array: PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354) [NBP3-07576] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PSMA/FOLH1/NAALADase I Mouse Monoclonal Antibody ...read more
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSMA/FOLH1/NAALADase I antibody (FOLH1/2354) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded horse brain stained with PSMA/FOLH1/NAALADase I antibody (FOLH1/2354). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded cat kidney stained with PSMA/FOLH1/NAALADase I antibody (FOLH1/2354). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354) [NBP3-07576] - Formalin-fixed, paraffin-embedded dog kidney stained with PSMA/FOLH1/NAALADase I antibody (FOLH1/2354). HIER: Tris/EDTA, pH9.0, ...read more

Product Details

Summary
Reactivity Hu, Ca, Eq, FeSpecies Glossary
Applications WB, IHC, MA
Clone
FOLH1/2354
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08385)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human PSMA/FOLH1/NAALADase I protein fragment (around aa 232-433) (exact sequence is proprietary) (Uniprot: Q04609 )
Localization
Cell Surface and Cytoplasmic
Marker
Prostate Epithelial Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOLH1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (FOLH1/2354)

  • Cell growth-inhibiting gene 27 protein
  • cell growth-inhibiting protein 27
  • EC 3.4.17.21
  • FGCP
  • folate hydrolase (prostate-specific membrane antigen) 1
  • Folate hydrolase 1
  • FOLH1
  • FOLHNAALADase I
  • Folylpoly-gamma-glutamate carboxypeptidase
  • GCP2
  • GCPII
  • GCPIImGCP
  • glutamate carboxylase II
  • glutamate carboxypeptidase 2
  • Glutamate carboxypeptidase II
  • Membrane glutamate carboxypeptidase
  • mopsm
  • NAALAD1
  • NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
  • NAALADase I
  • NAALAdase
  • N-acetylated alpha-linked acidic dipeptidase 1
  • prostate specific membrane antigen variant F
  • Prostate-specific membrane antigen
  • PSMA
  • PSMAFGCP
  • PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

PSMA, also known as Glutamate carboxypeptidase 2, is a 84kDa 750 amino acid protein that has 8 isoforms produced by alternative splicing. The primary function of this protein is to act as a glutamate carboxypeptidase. Current research on PSMA is being conducted in relation to prostate cancer, muscular atrophy, small cell carcinoma, coronary heart disease and Huntington's disease. PSMA is known to interact with MME, MTAP and RPS3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on PSMA/FOLH1/NAALADase I.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Bioinformatics

Gene Symbol FOLH1
Uniprot