![Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Protection of the retina against light damage in Grk1-/- mice subjected to BLE (12.5 klux for 0.5 hours) 1 week prior to imaging. Drug combination (M+B+T) or vehicle was injected i.p. 0.5 hours prior to induction of light damage (replicates are presented in Table 2). Representative flat-mount retina image of typical M- (green) and S-cone (blue) populations in vehicle-treated mouse retinas 1 week after BLE. Dashed white lines show border between damaged and healthy site in cone population. M+B+T at a dose 10-1-0.5 mg/kg maintained cone population at ~95%, whereas ONL thickness (representative of rod population) with this dose was decreased by ~50%. M+B+T 50-5-2.5 mg/kg dose led to full protection in both rod and cone population. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.19-26560) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-NB110-74730-img0011.jpg "Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Protection of the retina against light damage in Grk1-/- mice subjected to BLE (12.5 klux for 0.5 hours) 1 week prior to imaging. Drug combination (M+B+T) or vehicle was injected i.p. 0.5 hours prior to induction of light damage (replicates are presented in Table 2). Representative flat-mount retina image of typical M- (green) and S-cone (blue) populations in vehicle-treated mouse retinas 1 week after BLE. Dashed white lines show border between damaged and healthy site in cone population. M+B+T at a dose 10-1-0.5 mg/kg maintained cone population at ~95%, whereas ONL thickness (representative of rod population) with this dose was decreased by ~50%. M+B+T 50-5-2.5 mg/kg dose led to full protection in both rod and cone population. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.19-26560) licensed under a CC-BY license.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | OPN1MW |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30307502). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29162627). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | 0.02% Benzalkonium chloride |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Henri Leinonen |
Immunohistochemistry of intact specimen | Mouse | 07/18/2017 |
Summary
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reviewed by:
Verified Customer |
IHC-P | Mouse | 06/21/2016 |
Summary
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Enlarge |
reviewed by:
Verified Customer |
IHC | Mouse | 08/14/2012 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Opsin 1 (Medium Wave) Antibody (NB110-74730)Find related products by research area.
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| Henri Leinonen 07/18/2017 |
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| Application: | Immunohistochemistry of intact specimen | |
| Species: | Mouse |
| Verified Customer 06/21/2016 |
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| Application: | IHC-P | |
| Species: | Mouse |
| Verified Customer 08/14/2012 |
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| Application: | IHC | |
| Species: | Mouse |
![Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.](http://images.novusbio.com/fullsize/Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-Paraffin-NB110-74730-img0001.jpg "Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.")
![Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-NB110-74730-img0004.jpg "Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.")
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