![Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1](http://images.novusbio.com/fullsize/Prox1-Antibody-Western-Blot-NBP1-18605-img0007.jpg "Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Recombinant human Prox-1 |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PROX1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in IHC-Frozen reported in scientific literature (PMID 27122034). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 31696318). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.5X PBS, pH 7.2 |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein A purified |
| Reconstitution Instructions | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form. |
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reviewed by:
Verified Customer |
IF | Human | 11/06/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Prox1 Antibody (NBP1-18605)Find related products by research area.
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Read full blog post. |
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Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein) Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale?=?30 um. n?=?3 brains. E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale?=?30 um. n?=?3 brains. E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic ( red arrows) in the meninges but nuclear in neurons ( blue arrows). DAPI marks the nuclei in blue. Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00401-019-02091-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18605-img0008.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale?=?30 um. n?=?3 brains. E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale?=?30 um. n?=?3 brains. E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic ( red arrows) in the meninges but nuclear in neurons ( blue arrows). DAPI marks the nuclei in blue. Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00401-019-02091-z) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18605-img0006.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.")
![Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18605_rabbit-polyclonal-prox1-antibody-310202415293336.jpg "Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![N/A VEGF-D [HRP]](https://images.novusbio.com/images2/DATA_VEGFD_DVED00_ELISA_875.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")