Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 502 amino acids (MRGSGSHHHHHH-PDI) corresponding to Protein Disulfide Isomerase/P4HB Source: E. coli Amino Acid Sequence: MRGSGSHHHH HHAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL |
Details of Functionality | Thiol Protein Reductase Activity is 0.001 change in 650nm/ min-2, determined by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase Activity is 0.5 umol active RNase A min-1 umol PDIA2, according to the re-activation of reduced and denatured RNase A. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | P4HB |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of Protein Disulfide Isomerase/P4HB as determined by LAL method. |
Dilutions |
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Theoretical MW | 56.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for Protein Disulfide Isomerase/P4HB Recombinant Protein (NBP2-35195)Find related products by research area.
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Gene Symbol | P4HB |