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Protein Disulfide Isomerase/P4HB Antibody

 (6 Publications)
 (2 Reviews)
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Images

 
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Normal human brain.
Normal human brain.
ICC/IF Confocal analysis of 3T3 cells using P4HB antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 ...read more
IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of P4HB antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval ...read more
P4HB staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Protein Disulfide Isomerase/P4HB Antibody Summary

Immunogen
Purified full length P4hb isolated from rat liver.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:5000-1:20000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1-3 ug
  • Western Blot 1:10000-1:20000
Application Notes
This P4hb antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Control
Human Brain Frontal Lobe Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1921 in the following applications:
Publications
Read Publications using
NB100-1921 in the following applications:

  • ICC/IF
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protein Disulfide Isomerase/P4HB Antibody (NB100-1921)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for Protein Disulfide Isomerase/P4HB Antibody (NB100-1921) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-1921:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot Protein Disulfide Isomerase/P4HB NB100-1921
Enlarge 		5
reviewed by:
Verified Customer
 WB Rat 03/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
Immunocytochemistry Protein Disulfide Isomerase/P4HB NB100-1921
Enlarge 		5
reviewed by:
Bryan Tinsley
 ICC Mouse 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Protein Disulfide Isomerase/P4HB Antibody (NB100-1921). (Showing 1 - 1 of 1 FAQs).

  1. What permeabilization method was used to test this antibody?
    • For our protocol, our lab uses 0.1-0.5% Tween-20 in PBS or TBS for permeabilization.

Control Lysate(s)

Human Brain Frontal Lobe Membrane Tissue Lysate (Adult Membrane Normal)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
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4
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2
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1
0
Western Blot Protein Disulfide Isomerase/P4HB NB100-1921
Enlarge
 Verified Customer
03/06/2015
Application: WB
Species: Rat
read this review
Immunocytochemistry Protein Disulfide Isomerase/P4HB NB100-1921
Enlarge
 Bryan Tinsley
06/16/2014
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol P4HB
Entrez