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PPP2R1B Antibody

Images

 
Western Blot: PPP2R1B Antibody [NBP1-87251] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunocytochemistry/ Immunofluorescence: PPP2R1B Antibody [NBP1-87251] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal and non germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PPP2R1B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2R1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PPP2R1B Protein (NBP1-87251PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PPP2R1B Antibody

  • MGC26454
  • PP2A subunit A isoform PR65-beta
  • PP2A subunit A isoform R1-beta
  • PP2A, subunit A, PR65-beta isoform
  • PP2A, subunit A, R1-beta isoform
  • PP2A-Abeta
  • PR65B
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform
  • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
  • protein phosphatase 2, regulatory subunit A, beta
  • protein phosphatase 2, structural/regulatory subunit A, beta
  • serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A betaisoform

Background

The PPP2R1B gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric co

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP2R1B