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SDHD Antibody - BSA Free

Images

 
Western Blot: SDHD Antibody [NBP2-81948] - Analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: SDHD Antibody [NBP2-81948] - Analysis of SDHD in EL4 cells with SDHD antibody at 20 ug/ml.
Immunocytochemistry/ Immunofluorescence: SDHD Antibody [NBP2-81948] - Analysis of SDHD in EL4 cells with SDHD antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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SDHD Antibody - BSA Free Summary

Immunogen
SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD. Amino Acid Squence: HLSPSHHSGSKAASL
Specificity
At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
Predicted Species
Sheep (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHD
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/ml
  • Western Blot 1-2 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine: (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SDHD Antibody - BSA Free

  • CBT1
  • CII-4
  • cybS
  • PGL
  • PGL1
  • QPs3
  • SDH4
  • succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
  • Succinate dehydrogenase complex subunit D
  • succinate dehydrogenase complex, subunit D, integral membrane protein
  • succinate dehydrogenase ubiquinone cytochrome B small subunit
  • Succinate-ubiquinone oxidoreductase cytochrome b small subunit
  • Succinate-ubiquinone reductase membrane anchor subunit

Background

SDHD is part of Complex II. Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The subunit D protein is one of two integral membrane proteins anchoring the complex to the matrix side of the membrane. Mutations in SDHD have been linked to hereditary paraganglioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHD Antibody (NBP2-81948) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SDHD