![Western Blot: PPIL1 Antibody (08) [NBP3-06214] - Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution. Lane A: U87MG Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PPIL1-Antibody-08-Western-Blot-NBP3-06214-img0001.jpg "Western Blot: PPIL1 Antibody (08) [NBP3-06214] - Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution. Lane A: U87MG Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:19 kDa(We are unsure as to the identity of these extra bands.)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IP |
| Clone | 08 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPIL1 (Uniprot#: Q9Y3C6; Met1-Gly166). |
| Specificity | No cross-reactivity in ELISA with: E. coli cell lysate |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PPIL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PPIL1 |
![Flow Cytometry: PPIL1 Antibody (08) [NBP3-06214] - Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen (TM)), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/PPIL1-Antibody-08-Flow-Cytometry-NBP3-06214-img0002.jpg "Flow Cytometry: PPIL1 Antibody (08) [NBP3-06214] - Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen (TM)), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunoprecipitation: PPIL1 Antibody (08) [NBP3-06214] - PPIL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg U87MG Whole Cell Lysate 2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa](http://images.novusbio.com/fullsize/PPIL1-Antibody-08-Immunoprecipitation-NBP3-06214-img0003.jpg "Immunoprecipitation: PPIL1 Antibody (08) [NBP3-06214] - PPIL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg U87MG Whole Cell Lysate 2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa")
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