![Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Western-Blot-NB600-636-img0015.jpg "Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody is directed against mouse PPAR alpha/NR1C1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | PPAR alpha/NR1C1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha/NR1C1. (Uniprot: P23204) |
| Localization | Nuclear |
| Specificity | No reactivity is expected against other subtypes of PPAR. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPARA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Use in chromatin immunoprecipitation reported in scientific literature (PMID 26586378). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees . Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunocytochemistry-Immunofluorescence-NB600-636-img0021.jpg "Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees . Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.")
![Immunohistochemistry: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing PPARalpha (h-i) immunolabeling. White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a-i = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-NB600-636-img0022.jpg "Immunohistochemistry: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing PPARalpha (h-i) immunolabeling. White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a-i = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.")
![Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Western-Blot-NB600-636-img0014.jpg "Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunocytochemistry-Immunofluorescence-NB600-636-img0012.jpg "Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.")
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-Paraffin-NB600-636-img0006.jpg "Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).")
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-Paraffin-NB600-636-img0007.jpg "Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.")
![ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-ELISA-NB600-636-img0019.jpg "ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")