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Podocalyxin Like Antibody (3D3) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
3D3
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33108AF350
Alexa Fluor 405
NBP2-33108AF405
Alexa Fluor 488
NBP2-33108AF488
Alexa Fluor 532
NBP2-33108AF532
Alexa Fluor 594
NBP2-33108AF594
Alexa Fluor 647
NBP2-33108AF647
Alexa Fluor 700
NBP2-33108AF700
Alexa Fluor 750
NBP2-33108AF750
Allophycocyanin
NBP2-33108APC
Allophycocyanin/Cy7
NBP2-33108APCCY7
Biotin
NBP2-33108B
DyLight 350
NBP2-33108UV
DyLight 405
NBP2-33108V
DyLight 550
NBP2-33108R
DyLight 594
NBP2-33108DL594
DyLight 650
NBP2-33108C
DyLight 680
NBP2-33108FR
DyLight 755
NBP2-33108IR
FITC
NBP2-33108F
HRP
NBP2-33108H
Janelia Fluor 549
NBP2-33108JF549
Janelia Fluor 646
NBP2-33108JF646
PE
NBP2-33108PE
PE/Atto594
NBP2-33108PEATT594
PE/Cy5.5
NBP2-33108PECY55
PE/Cy7
NBP2-33108PECY7
PerCP
NBP2-33108PCP
Unconjugated
NBP2-25219
mFluor Violet 450 SE
NBP2-33108MFV450
mFluor Violet 500 SE
NBP2-33108MFV500
mFluor Violet 610 SE
NBP2-33108MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-25219
Azide and BSA Free
NBP2-33108
IHC-Prediluted
NBP2-45167
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Podocalyxin Like Antibody (3D3) [DyLight 488] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)
Localization
Cell surface and Cytoplasmic
Marker
Hematopoietic Stem Cell Marker
Specificity
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PODXL
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27771514).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Podocalyxin Like Antibody (3D3) [DyLight 488]

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PODXL