![Western Blot: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Western-Blot-NBP2-33108-img0010.jpg "Western Blot: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).")
| Reactivity | Hu, Mu, Rt, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | 3D3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-25219). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592) |
| Localization | Cell surface and Cytoplasmic |
| Marker | Hematopoietic Stem Cell Marker |
| Specificity | Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PODXL |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Podocalyxin Like Antibody (NBP2-33108)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PODXL |
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33108-img0009.jpg "Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.")
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33108-img0014.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).")
![Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108PE and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Flow-Intracellular-NBP2-33108-img0008.jpg "Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108PE and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the Azide and BSA Free form of this antibody.")
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33108-img0012.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).")
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33108-img0013.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).")
![Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody .](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33108-img0004.jpg "Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody .")
![Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108APC and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Azide-and-BSA-Free-Flow-Intracellular-NBP2-33108-img0007.jpg "Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108APC and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.")
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