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Mouse PLA2G1B ELISA Kit (Colorimetric)

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ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372] - Example standard curve for Mouse PLA2G1B ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372] - Example standard curve for Mouse PLA2G1B ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Serum
Standard Curve Range
2.82-180 pg/ml (example only; lot dependent)
Sensitivity
0.66 pg/ml

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Mouse PLA2G1B ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Mouse PLA2G1B.
Standard Curve Range
2.82-180 pg/ml (example only; lot dependent)
Sensitivity
0.66 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.9 %
Intra-Assay
CV% < 3.8
Spike Recovery
88-91%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PLA2G1B

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Mouse PLA2G1B ELISA Kit (Colorimetric)

  • Group IB phospholipase A2
  • Phosphatidylcholine 2-acylhydrolase 1B
  • phospholipase A2
  • phospholipase A2, group IB (pancreas)
  • PLA2
  • PLA2A
  • PLA2AMGC119834
  • PLA2EC 3.1.1.4
  • PLA2G1B
  • PPLA2
  • PPLA2MGC119835

Background

Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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NBP2-80372
Mouse PLA2G1B ELISA Kit (Colorimetric)
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Applications: ELISA

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Product PDFs

  Manual - Mouse PLA2G1B ELISA Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol PLA2G1B