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PIAS1 Antibody (3J2O8)

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Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477] - Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (NBP3-16477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477] - Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (NBP3-16477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477] - Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit mAb (NBP3-16477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
3J2O8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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PIAS1 Antibody (3J2O8) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 3-101 of human PIAS1 (O75925). DSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDG
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PIAS1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PIAS1 Antibody (3J2O8)

  • AR interacting protein
  • DDXBP1
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • PIAS1
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

PIAS1 encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIAS1