Periplakin Antibody Summary
| Immunogen |
The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493). |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
PPL |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunoprecipitation 2-5 mcg/mg lysate
- Western Blot 1:2000-1:10000
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
| Buffer |
TBS and 0.1% BSA |
| Preservative |
0.09% Sodium Azide |
| Concentration |
0.2 mg/ml |
| Purity |
Immunogen affinity purified |
Alternate Names for Periplakin Antibody
Background
Peroplakin is a component of the cornified envelope of keratinocytes. It may link the cornified envelope to desmosomes and intermediate filaments and may act as a localization signal in PKB/AKT-mediated signaling. Endogenous periplakin localizes to different cellular compartments, including plasma membrane, intermediate filament structures, the nucleus and mitochondria. Overexpression of the C-terminal part of periplakin, encompassing the PKB binding region, results in predominant intermediate filament localization and little nuclear staining. Periplakin is expressed in epithelial cells, together with envoplakin, the precursor of the epidermal cornified envelope. Furthermore, periplakin has been shown to be a target antigen in paraneoplastic pemphigus, and in keratinocytes it localizes at desmosomes and the interdesmosomal plasma membrane.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Secondary Antibodies
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Isotype Controls
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Blogs on Periplakin
![Western Blot: Periplakin Antibody [NB100-55264] - Detection of Mouse and Rat Periplakin/PPL by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody NB100-55264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Periplakin-Antibody-Western-Blot-NB100-55264-img0003.jpg "Western Blot: Periplakin Antibody [NB100-55264] - Detection of Mouse and Rat Periplakin/PPL by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody NB100-55264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Periplakin Antibody [NB100-55264] - Detection of Human Periplakin/PPL on HeLa whole cell lysate using NB100-55264. Periplakin/PPL was also immunoprecipitated by another rabbit anti-Periplakin/PPL antibody.](http://images.novusbio.com/fullsize/Periplakin-Antibody-Western-Blot-NB100-55264-img0001.jpg "Western Blot: Periplakin Antibody [NB100-55264] - Detection of Human Periplakin/PPL on HeLa whole cell lysate using NB100-55264. Periplakin/PPL was also immunoprecipitated by another rabbit anti-Periplakin/PPL antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")