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PDIA2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PDI Antibody [NBP2-49086] - Staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human lymph node.
Immunohistochemistry: PDI Antibody [NBP2-49086] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human colon shows low expression as expected.
Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human pancreas shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining in human pancreas and colon tissues using anti-PDIA2 antibody. Corresponding PDIA2 RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human kidney, liver, lymph node and pancreas using Anti-PDIA2 antibody NBP2-49086 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human liver.
Immunohistochemistry-Paraffin: PDI Antibody [NBP2-49086] - Staining of human kidney.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PDIA2 Antibody Summary

Immunogen
This antibody was developed against recombinant PDIA2 protein corresponding to amino acids: LIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRLFQQFGLTKDTV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PDIA2 Recombinant Protein Antigen (NBP2-49086PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDIA2 Antibody

  • Pancreas-specific protein disulfide isomerase
  • pancreatic protein disulfide isomerase
  • PDA2
  • PDI
  • PDIp
  • PDIR
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide-isomerase A2
  • Rho GDP dissociation inhibitor gamma

Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDIA2