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Perilipin Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Fi, Bv, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MS
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-40760AF350
Alexa Fluor 405
NB110-40760AF405
Alexa Fluor 488
NB110-40760AF488
Alexa Fluor 532
NB110-40760AF532
Alexa Fluor 594
NB110-40760AF594
Alexa Fluor 647
NB110-40760AF647
Alexa Fluor 700
NB110-40760AF700
Alexa Fluor 750
NB110-40760AF750
Allophycocyanin
NB110-40760APC
Biotin
NB110-40760B
CoraFluor 1
NB110-40760CL1
DyLight 350
NB110-40760UV
DyLight 405
NB110-40760V
DyLight 488
NB110-40760G
DyLight 550
NB110-40760R
DyLight 594
NB110-40760DL594
DyLight 650
NB110-40760C
DyLight 680
NB110-40760FR
DyLight 755
NB110-40760IR
HRP
NB110-40760H
Janelia Fluor 525
NB110-40760JF525
Janelia Fluor 549
NB110-40760JF549
Janelia Fluor 585
NB110-40760JF585
Janelia Fluor 635
NB110-40760JF635
Janelia Fluor 646
NB110-40760JF646
Janelia Fluor 669
NB110-40760JF669
PE
NB110-40760PE
PerCP
NB110-40760PCP
Unconjugated
NB110-40760
mFluor Violet 450 SE
NB110-40760MFV450
mFluor Violet 500 SE
NB110-40760MFV500
mFluor Violet 610 SE
NB110-40760MFV610
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Perilipin Antibody [FITC] Summary

Immunogen
This Perilipin Antibody was developed against a synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]
Localization
Endoplasmic reticulum, Lipid droplet (surface-associated).
Predicted Species
Bovine (92%), Sheep (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Imaging Mass Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 27012897).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Perilipin Antibody [FITC]

  • PERI
  • perilipin 1
  • Perilipin
  • perilipin-1
  • PLIN
  • PLIN1
  • PLINLipid droplet-associated protein

Background

Perilipin is a member of the perilipin or PAT family which includes perilipin or perilipin 1 (PLIN1), ADRP/perilipin 2, TIP47/perilipin 3, S3-12/perilipin 4, and LSDP5/perilipin 5 (also called OXPAT). PLIN1 is localized on lipid droplet surfaces in adipocytes and steroidogenic cells wherein it plays a key role in basal/stimulated lipid storage as well as breakdown. Under basal conditions, perilipin protects the lipid core from lipases (e.g. hormone-sensitive lipase and adipocyte TAG lipase) and co-activator CGI-58 by sequestering the enzymes from the lipid droplet surfaces. However, upon beta-adrenergic stimulation, PKA phosphorylates PLIN1 and causes the release of CGI-58 so it can bind and stimulate ATGL and also allows/recruits HSL to translocate to the lipid droplet surface. PLIN1 knockout increases basal lipolysis and decreases LD size in adipocytes and causes resistance to diet induced obesity in mice. In humans, lower PLIN1 expression is associated with higher rates of lipolysis and mutation in PLIN1 or its expression in obese human adipose tissue, correlates positively with insulin sensitivity, and have been established as the cause of familial partial lipodystrophy type 4 (FPLD4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BSA Free
NB110-60509
Western Blot OXPAT Antibody - BSA Free
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Species: Mu
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin Antibody (NB110-40760F). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody conjugated with FITC work even for Immunofluorescence assay?
    • The FITC conjugate of this perilipin antibody, NB110-40760F, has not itself been tested in ICC/IF yet, which is why this application is not stated on its datasheet. Nonetheless, the unconjugated format of the antibody, NB110-40760, has, and so is guaranteed for ICC/IF. Our product notes for this antibody say that use in ICC/IF was reported in the literature, in this paper: http://www.ncbi.nlm.nih.gov/pubmed/18201980) Therefore, we cannot guarantee NB110-40760F for IF, but the evidence we do have does indicate that it should work for you. If you did go ahead and test NB110-40760F in IF, you would be eligible for our Innovator's Reward, where in return for sharing your findings with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product (please see here for further details: Innovator's Reward program).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLIN1