Abhd5 Antibody (1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 5 applications: Block/Neutralize, ICC/IF, IF/IHC, KO, WB.

Showing Publications 1 - 10 of 33. Show All 33 Publications. Collapse Publications.

Publications using H00051099-M01	Applications	Species
Gandhi AY, Yu J, Gupta A et al. Cytokine-Mediated STAT3 Transcription Supports ATGL/CGI-58-Dependent Adipocyte Lipolysis in Cancer Cachexia Frontiers in Oncology 2022-06-15 [PMID: 35785158] (Block/Neutralize)	Block/Neutralize
Ivan B, Katharina K, Sophie H et al. Off-target effects of the lysosomal acid lipase inhibitors Lalistat-1 and Lalistat-2 on neutral lipid hydrolases. Mol Metab. 2022-04-30 [PMID: 35504532]
Talbott HA, Plewes MR, Krause C et al. Formation and characterization of lipid droplets of the bovine corpus luteum Sci Rep 2020-07-09 [PMID: 32647143] (WB, Bovine)	WB	Bovine
Gabrielle V, Isabelle R, Arnaud C et al. The ATGL Lipase Cooperates With ABHD5 to Mobilize Lipids for Hepatitis C Virus Assembly. PLoS Pathog. 2020-06-15 [PMID: 32542055]
Wagner C, Hois V, Pajed L et al. Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation. Biochim Biophys Acta Mol Cell Biol Lipids . 2020-05-01 [PMID: 32361002]
Yang P, Wang Y, Tang W et al. Western diet induces severe nonalcoholic steatohepatitis, ductular reaction, and hepatic fibrosis in liver CGI-58 knockout mice. Sci Rep. 2020-03-13 [PMID: 32170127]
Alexander Y, Emilio M, Ljiljana M et al. Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes. Nat Metab. 2019-05-06 [PMID: 31497752]
Shang S, Ji X, Zhang L et al. Macrophage ABHD5 suppresses NF-kappa B-dependent matrix metalloproteinase expression and cancer metastasis Cancer Res. 2019-08-22 [PMID: 31439546] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Korbelius M, Vujic N, Sachdev V et al. ATGL/CGI-58-Dependent Hydrolysis of a Lipid Storage Pool in Murine Enterocytes. Cell Rep. 2019-08-13 [PMID: 31412256]
Ou J, Peng Y, Yang W et al. ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield. Nat Commun. 2019-03-06 [PMID: 30842415]
Ou J, Miao H, Ma Y et al. Loss of Abhd5 Promotes Colorectal Tumor Development and Progression by Inducing Aerobic Glycolysis and Epithelial-Mesenchymal Transition. Cell Rep 2018-09-04 [PMID: 30184511]
Bhavya S, Lew PS, Mizuno TM. Stimulation of white adipose tissue lipolysis by xenin, a neurotensin-related peptide. Biochem Biophys Res Commun 2018-04-15 [PMID: 29530527] (Mouse)		Mouse
Shin H, Ma Y, Chanturiya T et al. Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice. Cell Metab 2017-10-05 [PMID: 28988822]
Bhavya S, Lew PS, Mizuno TM. Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue. Neuropeptides 2017-02-09 [PMID: 28190525]
Peng Y, Miao H, Wu S et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy 2016-11-01 [PMID: 27559856] (KO, Mouse)	KO	Mouse
Miao H, Ou J, Peng Y et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun 2016-05-18 [PMID: 27189574] (WB)	WB
Vieyres G, Welsch K, Gerold G et al. ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production. PLoS Pathog 2016-04-01 [PMID: 27124600] (WB)	WB
Heier C, Radner FP, Moustafa T et al. G0/G1 switch gene-2 regulates cardiac lipolysis. J Biol Chem. 2015-09-08 [PMID: 26350455]
Hofer P, Boeszoermenyi A, Jaeger D et al. Fatty acid-binding proteins interact with comparative gene identification-58 linking lipolysis with lipid ligand shuttling. J Biol Chem. 2015-05-07 [PMID: 25953897]
Das SK, Stadelmeyer E, Schauer S et al. Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58. Int J Mol Sci 2015-01-01 [PMID: 25894224] (ICC/IF)	ICC/IF
Bakke SS, Feng YZ, Nikolic N et al. Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects. PLoS One 2015-01-01 [PMID: 25790476] (WB, Human)	WB	Human
Xie P, Kadegowda AK, Ma Y et al. Muscle-Specific Deletion of Comparative Gene Identification-58 (CGI-58) Causes Muscle Steatosis but Improves Insulin Sensitivity in Male Mice. Endocrinology. 2015-03-09 [PMID: 25751639] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Camus G, Schweiger M, Herker E et al. The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets. J Biol Chem. 2014-12-26 [PMID: 25381252]
Patel Satish, Yang Wei, Kozusko Kristina et al. Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. Proc Natl Acad Sci U S A. 2014-06-24 [PMID: 24927580] (WB, Mouse)	WB	Mouse
Kurtz C Lisa, Peck Bailey C E, Fannin Emily E et al. MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes. Diabetes. 2014-04-10 [PMID: 24722248]
Louche K, Badin PM, Montastier E et al. Endurance Exercise Training Up-Regulates Lipolytic Proteins and Reduces Triglyceride Content in Skeletal Muscle of Obese Subjects. J Clin Endocrinol Metab. 2013-10-31 [PMID: 24178794]
Inoue T, Kobayashi K, Inoguchi T et al. Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice. Biochem Biophys Res Commun. 2013-07-22 [PMID: 23886955]
Guo F, Ma Y, Kadegowda AK et al. Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice. J Lipid Res. 2013-08-01 [PMID: 23733885] (Mouse)		Mouse
Pollak NM, Schweiger M, Jaeger D et al. Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier. J Lipid Res. 2013-04-01 [PMID: 23345410] (Mouse)		Mouse
Grall A, Guaguere E, Planchais S et al. PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans. Nat Genet. 2012-01-15 [PMID: 22246504]
Caviglia JM, Betters JL, Dapito D-H et al. Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity. J Lipid Res. 2011-08-03 [PMID: 21885429]
Stenson BM, Ryden M, Venteclef N et al. Liver X Receptor (LXR) Regulates Human Adipocyte Lipolysis. J Biol Chem. 2010-10-28 [PMID: 21030586]
Kinney BP, Qiao L, Levaugh JM et al. B56{alpha}/Protein Phosphatase 2A Inhibits Adipose Lipolysis in High-Fat Diet-Induced Obese Mice. Endocrinology. 2010-06-09 [PMID: 20534721]
Show All 33 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Heather Talbott	WB	Bovine, Human, Mouse	09/12/2017	View Summary Application Western Blot Sample Tested bovine adipose tissue lysate,bovine liver tissue lysate,HeLa Cytosol,Hela whole cell lysate,Mouse adipose tissue,mouse liver lysate Species Bovine, Human, Mouse Lot H5091-1F3 Comments Comments 4-20% gradient gel 15 ug/well PVDF and Nitocellulose Block: 5% NFDM in TBS 0.1% Tween-20 Primary: 1:500 dilution, 1% NFDM in TBS 0.1% Tween-20, overnight at 4 C Secondary: 1:10,000 anti-mouse - HRP (Jackson ImmunoResearch 115-035-205); 1:5000 anti-biotin - HRP (Cell Signaling 7075P5), 1% NFDM in TBS 0.1% Tween-20, 1 hr at RT ECL: SuperSignal West Femto (ThermoFisher Scientific 34096), UVP Image Station

Array H00051099-M01

• Abhd5 Antibodies
• Abhd5 Lysate
• Abhd5 Proteins