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Adiponutrin/PNPLA3 Antibody [Unconjugated]

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Adiponutrin/ADPN was detected in immersion fixed human adipocytes using 10 µg/mL Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Adiponutrin/ADPN was detected in immersion fixed human adipocytes using 10 µg/mL Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) for 3 hours at room temperature. Cells ...read more
Western blot shows lysates of human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5208-SP
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Adiponutrin/PNPLA3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Adiponutrin/PNPLA3
Arg160-Arg349
Accession # Q9NST1
Specificity
Detects human Adiponutrin/PNPLA3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PNPLA3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5208 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponutrin/PNPLA3 Antibody [Unconjugated]

  • Acylglycerol O-acyltransferase
  • Adiponutrin
  • ADPN
  • ADPNdJ796I17.1
  • C22orf20
  • Calcium-independent phospholipase A2-epsilon
  • chromosome 22 open reading frame 20
  • EC 2.3.1.-
  • EC 2.7.7.56
  • EC 3.1.1.3
  • EC 4.2.3.4
  • FLJ22012
  • iPLA(2)epsilon
  • iPLA2-epsilon
  • patatin-like phospholipase domain containing 3
  • patatin-like phospholipase domain-containing protein 3
  • PNPLA3

Background

Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adiponutrin/PNPLA3 Antibody (AF5208)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol PNPLA3
Uniprot